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. 2012 Mar 1;139(5):991–1000. doi: 10.1242/dev.077255

Fig. 1.

Fig. 1.

Sine oculis and Optix have limited intrinsic ability to activate transcription. (A-C) Confocal images of third instar larval eye-antennal discs. cb49-GAL4 drives expression of the GFP reporter at low levels in all cells of the eye-antennal disc. Arrows in B and C mark the location of ectopic eyes. (D) Transcriptional activation of UAS-lacZ in yeast by Optix-FL, DSix4-FL, So-FL, So-ΔNT, So-ΔSD, So-ΔHD and So-ΔCT. Asterisks indicate that So-ΔSD and So-ΔCT failed to activate transcription of the UAS-lacZ reporter. (E) Transcriptional activation of UAS-HIS3 by So, Ey and Eya in yeast. Assay is carried out in the presence of increasing amounts of the 3AT histidine biosynthesis inhibitor to determine the relative strength of activation potential. (F) Schematic of So full-length and deletion constructs.