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Transcriptional repressor forms of So and Optix initiate ectopic eye formation. (A,E) Schematic of So-VP16, So-EN, Optix-VP16 and Optix-EN constructs used in this study. (B,F) Immunoblot (B) and western blot (F) demonstrating that So-VP16 and Optix-VP16 proteins are made in S2 cells and Drosophila embryos. (C,D,G,H) Scanning electron microscopy and confocal images of ectopic eyes (arrows) formed by expression of So-EN and Optix-EN.
