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Formation of SIX-Eya and SIX-Gro transcriptional complexes. Immunoblots of biochemical interactions from Kc167 cells between the Drosophila SIX proteins, Eya and Gro. Production of individual proteins is shown in the nuclear lysate (NL) lanes. Successful isolation of individual proteins is shown in the immunoprecipitation (IP) lanes. Specificity of the pull-downs is shown in the mock IP lanes. Protein interactions are shown in the immunoblot (IB) lanes.
