Abstract
Supercoiled mitochondrial DNA (mtDNA) has been isolated in preparative amounts from five different plant species and compared on agarose gels. The gels reveal considerable mtDNA size heterogeneity within each species as well as substantial differences in the range and frequency of the mtDNA size classes among the different plants. The two lowest size classes of three of the plants have been extracted and their molecular weights determined. Comparison of two N. tabacum W38 culture lines has revealed significant differences in their mtDNA size classes. However, despite the totally different supercoil patterns, these two lines have almost identical restriction digests. The availability of preparative amounts of separated mtDNA size classes will make it possible to carry out a detailed analysis of plant mitochondrial genomes.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Cummings D. J., Belcour L., Grandchamp C. Mitochondrial DNA from Podospora anserina. II. Properties of mutant DNA and multimeric circular DNA from senescent cultures. Mol Gen Genet. 1979 Mar 27;171(3):239–250. doi: 10.1007/BF00267578. [DOI] [PubMed] [Google Scholar]
- Gamborg O. L., Miller R. A., Ojima K. Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res. 1968 Apr;50(1):151–158. doi: 10.1016/0014-4827(68)90403-5. [DOI] [PubMed] [Google Scholar]
- Hollenberg C. P., Borst P., van Bruggen E. F. Mitochondrial DNA. V. A 25 micron closed circular duplex DNA molecule in wild-type yeast mitochondria. Stucture and genetic complexity. Biochim Biophys Acta. 1970 May 21;209(1):1–15. [PubMed] [Google Scholar]
- Levings C. S., 3rd, Pring D. R. Restriction endonuclease analysis of mitochondrial DNA from normal and Texas cytoplasmic male-sterile maize. Science. 1976 Jul 9;193(4248):158–160. doi: 10.1126/science.193.4248.158. [DOI] [PubMed] [Google Scholar]
- Synenki R. M., Levings C. S., Shah D. M. Physicochemical characterization of mitochondrial DNA from soybean. Plant Physiol. 1978 Mar;61(3):460–464. doi: 10.1104/pp.61.3.460. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Thuring R. W., Sanders J. P., Borst P. A freeze-squeeze method for recovering long DNA from agarose gels. Anal Biochem. 1975 May 26;66(1):213–220. doi: 10.1016/0003-2697(75)90739-3. [DOI] [PubMed] [Google Scholar]