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. 1981 Sep 25;9(18):4669–4676. doi: 10.1093/nar/9.18.4669

Nucleotide sequence of the asnA gene coding for asparagine synthetase of E. coli K-12.

M Nakamura, M Yamada, Y Hirota, K Sugimoto, A Oka, M Takanami
PMCID: PMC327466  PMID: 6117826

Abstract

We have subcloned the asnA gene of E. coli K-12, a gene coding for asparagine synthetase, from a previously cloned 6 mega-dalton segment of E. coli chromosome containing the DNA replication origin, ori, and asnA. The complete nucleotide sequence of the asnA gene was determined: the region of the structural gene extends 990 base-pairs at nucleotide positions 1434-2423 (see Fig. 3), which codes for a polypeptide of 330 amino-acid residues with a molecular weight of 36,688 daltons. The nucleotide sequences of the promoter and the ribosome-binding site of the gene are also assigned. We discuss the properties of its polypeptide.

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Selected References

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