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. 1981 Oct 10;9(19):5163–5174. doi: 10.1093/nar/9.19.5163

The primary structure of a plant storage protein: zein.

D Geraghty, M A Peifer, I Rubenstein, J Messing
PMCID: PMC327507  PMID: 6895552

Abstract

The protein sequence of a representative of the zeins, the major storage proteins of maize, has been derived from the nucleotide sequence of a zein cDNA clone. This cDNA was sequence both by the Maxam and Gilbert and the M13-dideoxy techniques. The nucleotide sequence encompasses the non-translated 3' terminus of the mRNA, the entire coding sequence specifying both the mature zein protein and a small signal peptide, and a portion of the non-translated 5' region. The deduced amino acid composition and the amino-terminal amino acid sequence closely resemble those derived from chemical analysis of the zein protein fraction. The data presented represent the first complete amino acid sequence of a plant storage protein.

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Selected References

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