Skip to main content
PMC home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1981 Nov 11;9(21):5747–5761. doi: 10.1093/nar/9.21.5747

DNA-like duplexes with repetitions. III. Efficient template-guided chemical polymerization of d(TGGCCAAGCTp).

Z A Shabarova, N G Dolinnaya, V L Drutsa, N P Melnikova, A A Purmal
PMCID: PMC327558  PMID: 6273807

Abstract

Self-association of a decanucleotide d(TGGCCAAGCTp) in an aqueous solution is shown by UV spectroscopy, CD and sedimentation analysis to yield a pseudopolymeric (concatemeric) duplex having a geometry similar to that of DNA B-type. It is demonstrated that in conditions when the concatemeric duplex is stable a water-soluble carbodiimide induces efficient polymerization of the 3'- or 5'-phosphorylated decanucleotide, and the resulting polymers d(TGGCCAAGCTp)2-10 contain only natural phosphodiester bonds. In conditions optimal for template-guided polymerization of d(TGGCCAAGCTp) the overall yield of 20-100-member polynucleotides exceeds 90%. The obtained polymeric duplexes are cleaved by restriction endonuclease Alu II, Bsu RI, and Hind III to corresponding decamers which were isolated and sequenced.

Full text

PDF

Images in this article

5756Image
on p.5756

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ambartsumyan N. S., Mazo A. M. Elimination of the secondary structure effect in gell sequencing of nucleic acids. FEBS Lett. 1980 Jun 2;114(2):265–268. doi: 10.1016/0014-5793(80)81130-6. [DOI] [PubMed] [Google Scholar]
  2. Augusti-Tocco G., Brown G. L. Reaction of N-cyclohexyl, N'-beta (4-methylmorpholinium) ethyl carbodiimide iodide with nucleic acids and polynucleotides. Nature. 1965 May 15;206(985):683–685. doi: 10.1038/206683a0. [DOI] [PubMed] [Google Scholar]
  3. Borer P. N., Dengler B., Tinoco I., Jr, Uhlenbeck O. C. Stability of ribonucleic acid double-stranded helices. J Mol Biol. 1974 Jul 15;86(4):843–853. doi: 10.1016/0022-2836(74)90357-x. [DOI] [PubMed] [Google Scholar]
  4. Borer P. N., Uhlenbeck O. C., Dengler B., Tinoco I., Jr Circular dichroism of double-helical oligoribonucleotides. J Mol Biol. 1973 Nov 15;80(4):759–771. doi: 10.1016/0022-2836(73)90208-8. [DOI] [PubMed] [Google Scholar]
  5. Kirsch D. R., Cohen E. H. Restriction endonuclease analysis and nucleotide composition of satellite III DNA from Drosophila virilis. Biochem Biophys Res Commun. 1980 Oct 31;96(4):1603–1609. doi: 10.1016/0006-291x(80)91357-1. [DOI] [PubMed] [Google Scholar]
  6. Marck C., Guschlbauer W. A simple method for the computation of first neighbour frequencies of DNAs from CD spectra. Nucleic Acids Res. 1978 Jun;5(6):2013–2031. doi: 10.1093/nar/5.6.2013. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Martin F. H., Uhlenbeck O. C., Doty P. Self-complementary oligoribonucleotides: adenylic acid-uridylic acid block copolymers. J Mol Biol. 1971 Apr 28;57(2):201–215. doi: 10.1016/0022-2836(71)90341-x. [DOI] [PubMed] [Google Scholar]
  8. Mills D. R., Kramer F. R. Structure-independent nucleotide sequence analysis. Proc Natl Acad Sci U S A. 1979 May;76(5):2232–2235. doi: 10.1073/pnas.76.5.2232. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Naylor R., Gilham P. T. Studies on some interactions and reactions of oligonucleotides in aqueous solution. Biochemistry. 1966 Aug;5(8):2722–2728. doi: 10.1021/bi00872a032. [DOI] [PubMed] [Google Scholar]
  10. Pöschl E., Streeck R. E. Prototype sequence of bovine 1.720 satellite DNA. J Mol Biol. 1980 Oct 15;143(1):147–153. doi: 10.1016/0022-2836(80)90128-x. [DOI] [PubMed] [Google Scholar]
  11. STUDIER F. W. SEDIMENTATION STUDIES OF THE SIZE AND SHAPE OF DNA. J Mol Biol. 1965 Feb;11:373–390. doi: 10.1016/s0022-2836(65)80064-x. [DOI] [PubMed] [Google Scholar]
  12. Sealy L., Hartley J., Donelson J., Chalkley R., Hutchison N., Hamkalo B. Characterization of a highly repetitive sequence DNA family in rat. J Mol Biol. 1981 Jan 15;145(2):291–318. doi: 10.1016/0022-2836(81)90207-2. [DOI] [PubMed] [Google Scholar]
  13. Shabarova Z. A., Dolinnaya N. G., Turkin S. I., Gromova E. S. DNA-like duplexes with repetitions. I. Properties of concatemer duplexes formed by d(T-G-C-A-C-A-T-G). Nucleic Acids Res. 1980 Jun 11;8(11):2413–2429. doi: 10.1093/nar/8.11.2413. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Shabarova Z. A., Prokofiev M. A. A model of enzymatic synthesis of the internucleotide bond between oligodeoxynucleotides. FEBS Lett. 1970 Dec 11;11(4):237–240. doi: 10.1016/0014-5793(70)80537-3. [DOI] [PubMed] [Google Scholar]
  15. Shabarova Z. A. The application of solid phase method-synthesized oligodeoxyribonucleotides to molecular biology problems. Nucleic Acids Symp Ser. 1980;(7):259–279. [PubMed] [Google Scholar]
  16. Uesugi S., Ts'o P. O. Chemical polymerization of oligonucleotides directed by a complementary polynucleotide. Preparation and polymerization of oligo(2'-O-methylinosine 3'-phosphate). Biochemistry. 1974 Jul 16;13(15):3142–3152. doi: 10.1021/bi00712a022. [DOI] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES