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. 1981 Dec 11;9(23):6293–6304. doi: 10.1093/nar/9.23.6293

Biosynthesis and transport of yeast mitochondrial phenylalanyl-tRNA synthetase.

M Diatewa, A J Stahl
PMCID: PMC327604  PMID: 7033932

Abstract

The biosynthesis of yeast mitochondrial Phe-tRNA synthetase is studied in vivo. Antibodies against the enzyme are raised in rabbits. They precipitate two proteins in the post-ribosomal supernatant of the yeast cell homogenate. Immunoprecipitate analysis on SDS - gel electrophoresis shows that the two types of mitochondrial enzyme subunits with molecular weights of 57,000 and 72,000, respectively, are cytoplasmically synthesized as larger, individual precursors. Terminal extensions of the precursors prevent enzyme activity. Mitochondrial membranes linked protease(s) play(s) an active role in maturation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ades I. Z., Butow R. A. The products of mitochondria-bound cytoplasmic polysomes in yeast. J Biol Chem. 1980 Oct 25;255(20):9918–9924. [PubMed] [Google Scholar]
  2. Ades I. Z., Butow R. A. The transport of proteins into yeast mitochondria. Kinetics and pools. J Biol Chem. 1980 Oct 25;255(20):9925–9935. [PubMed] [Google Scholar]
  3. Ames G. F., Nikaido K. Two-dimensional gel electrophoresis of membrane proteins. Biochemistry. 1976 Feb 10;15(3):616–623. doi: 10.1021/bi00648a026. [DOI] [PubMed] [Google Scholar]
  4. Cashmore A. R., Broadhurst M. K., Gray R. E. Cell-free synthesis of leaf protein: Identification of an apparent precursor of the small subunit of ribulose-1,5-bisphosphate carboxylase. Proc Natl Acad Sci U S A. 1978 Feb;75(2):655–659. doi: 10.1073/pnas.75.2.655. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Chua N. H., Schmidt G. W. Post-translational transport into intact chloroplasts of a precursor to the small subunit of ribulose-1,5-bisphosphate carboxylase. Proc Natl Acad Sci U S A. 1978 Dec;75(12):6110–6114. doi: 10.1073/pnas.75.12.6110. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Conboy J. G., Kalousek F., Rosenberg L. E. In vitro synthesis of a putative precursor of mitochondrial ornithine transcarbamoylase. Proc Natl Acad Sci U S A. 1979 Nov;76(11):5724–5727. doi: 10.1073/pnas.76.11.5724. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Diatewa M., Stahl A. J. Purification and subunit structure of mitochondrial phenylalanyl-tRNA synthetase from yeast. Biochem Biophys Res Commun. 1980 May 14;94(1):189–198. doi: 10.1016/s0006-291x(80)80205-1. [DOI] [PubMed] [Google Scholar]
  8. Diatewa M., Viard I., Stahl A. J. Obtention de protoplastes de levure à l'aide de différences préparations de suc hépato-pancréatique d'Helix pomatia. Biochimie. 1981 Jan;63(1):67–69. doi: 10.1016/s0300-9084(81)80148-4. [DOI] [PubMed] [Google Scholar]
  9. Dobberstein B., Blobel G., Chua N. H. In vitro synthesis and processing of a putative precursor for the small subunit of ribulose-1,5-bisphosphate carboxylase of Chlamydomonas reinhardtii. Proc Natl Acad Sci U S A. 1977 Mar;74(3):1082–1085. doi: 10.1073/pnas.74.3.1082. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Elder J. H., Pickett R. A., 2nd, Hampton J., Lerner R. A. Radioiodination of proteins in single polyacrylamide gel slices. Tryptic peptide analysis of all the major members of complex multicomponent systems using microgram quantities of total protein. J Biol Chem. 1977 Sep 25;252(18):6510–6515. [PubMed] [Google Scholar]
  11. Engvall E., Perlmann P. Enzyme-linked immunosorbent assay, Elisa. 3. Quantitation of specific antibodies by enzyme-labeled anti-immunoglobulin in antigen-coated tubes. J Immunol. 1972 Jul;109(1):129–135. [PubMed] [Google Scholar]
  12. Fairbanks G., Steck T. L., Wallach D. F. Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane. Biochemistry. 1971 Jun 22;10(13):2606–2617. doi: 10.1021/bi00789a030. [DOI] [PubMed] [Google Scholar]
  13. Faye G., Kujawa C., Fukuhara H. Physical and genetic organization of petite and grande yeast mitochondrial DNA. IV. In vivo transcription products of mitochondrial DNA and localization of 23 S ribosomal RNA in petite mutants of saccharomyces cerevisiae. J Mol Biol. 1974 Sep 5;88(1):185–203. doi: 10.1016/0022-2836(74)90304-0. [DOI] [PubMed] [Google Scholar]
  14. Harmey M. A., Neupert W. Biosynthesis of mitochondrial citrate synthase in Neurospora crassa. FEBS Lett. 1979 Dec 15;108(2):385–389. doi: 10.1016/0014-5793(79)80569-4. [DOI] [PubMed] [Google Scholar]
  15. Kern D., Dietrich A., Fasiolo F., Renaud M., Giegé R., Ebel J. P. The yeast aminoacyl-tRNA synthetases. Methodology for their complete or partial purification and comparison of their relative activities under various extraction conditions. Biochimie. 1977;59(5-6):453–462. doi: 10.1016/s0300-9084(77)80050-3. [DOI] [PubMed] [Google Scholar]
  16. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  17. Lewin A. S., Gregor I., Mason T. L., Nelson N., Schatz G. Cytoplasmically made subunits of yeast mitochondrial F1-ATPase and cytochrome c oxidase are synthesized as individual precursors, not as polyproteins. Proc Natl Acad Sci U S A. 1980 Jul;77(7):3998–4002. doi: 10.1073/pnas.77.7.3998. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Maccecchini M. L., Rudin Y., Blobel G., Schatz G. Import of proteins into mitochondria: precursor forms of the extramitochondrially made F1-ATPase subunits in yeast. Proc Natl Acad Sci U S A. 1979 Jan;76(1):343–347. doi: 10.1073/pnas.76.1.343. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Maccecchini M. L., Rudin Y., Schatz G. Transport of proteins across the mitochondrial outer membrane. A precursor form of the cytoplasmically made intermembrane enzyme cytochrome c peroxidase. J Biol Chem. 1979 Aug 25;254(16):7468–7471. [PubMed] [Google Scholar]
  20. Mihara K., Blobel G. The four cytoplasmically made subunits of yeast mitochondrial cytochrome c oxidase are synthesized individually and not as a polyprotein. Proc Natl Acad Sci U S A. 1980 Jul;77(7):4160–4164. doi: 10.1073/pnas.77.7.4160. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Sakakibara R., Huynh Q. K., Nishida Y., Watanabe T., Wada H. In vitro synthesis of glutamic oxaloacetic transaminase isozymes of rat liver. Biochem Biophys Res Commun. 1980 Aug 29;95(4):1781–1788. doi: 10.1016/s0006-291x(80)80105-7. [DOI] [PubMed] [Google Scholar]
  22. Schmidt G. W., Devillers-Thiery A., Desruisseaux H., Blobel G., Chua N. H. NH2-terminal amino acid sequences of precursor and mature forms of the ribulose-1,5-bisphosphate carboxylase small subunit from Chlamydomonas reinhardtii. J Cell Biol. 1979 Dec;83(3):615–622. doi: 10.1083/jcb.83.3.615. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Schneller J. M., Martin R., Stahl A., Dirheimer G. Studies of odd bases in yeast mitochondrial tRNA: absense of the fluorescent "Y" base in mitochondrial DNA coded tRNAPhe, absence of 4-thiouridine. Biochem Biophys Res Commun. 1975 Jan 2;64(3):1046–1053. doi: 10.1016/0006-291x(75)90153-9. [DOI] [PubMed] [Google Scholar]
  24. Schneller J. M., Schneller C., Martin R., Stahl A. J. Nuclear origin of specific yeast mitochondrial aminoacyl-tRNA synthetases. Nucleic Acids Res. 1976 May;3(5):1151–1165. doi: 10.1093/nar/3.5.1151. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Shore G. C., Carignan P., Raymond Y. In vitro synthesis of a putative precursor to the mitochondrial enzyme, carbamyl phosphate synthetase. J Biol Chem. 1979 May 10;254(9):3141–3144. [PubMed] [Google Scholar]
  26. Sonderegger P., Jaussi R., Christen P. Cell-free synthesis of a putative precursor of mitochondrial aspartate aminotransferase with higher molecular weight. Biochem Biophys Res Commun. 1980 Jun 30;94(4):1256–1260. doi: 10.1016/0006-291x(80)90554-9. [DOI] [PubMed] [Google Scholar]
  27. Yamauchi K., Hayashi N., Kikuchi G. Translocation of delta-aminolevulinate synthase from the cytosol to the mitochondria and its regulation by hemin in the rat liver. J Biol Chem. 1980 Feb 25;255(4):1746–1751. [PubMed] [Google Scholar]
  28. Zimmermann R., Paluch U., Neupert W. Cell-free synthesis of cytochrome c. FEBS Lett. 1979 Dec 1;108(1):141–146. doi: 10.1016/0014-5793(79)81196-5. [DOI] [PubMed] [Google Scholar]

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