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. 1981 Dec 11;9(23):6421–6428. doi: 10.1093/nar/9.23.6421

Primary structure of an unusual glycine tRNA UGA suppressor.

N E Prather, E J Murgola, B H Mims
PMCID: PMC327613  PMID: 7033934

Abstract

We have determined the nucleotide sequences of two UGA-suppressing glycine transfer RNAs. The suppressor tRNAs were previously shown to translate both UGA and UGG and to have arisen as a consequence of mutation in glyT, the gene for the GGA/G-reading glycine tRNA of Escherichia coli. In each mutant tRNA, the primary sequence change was the substitution of adenine for cytosine in the 3' position of the anticodon. In addition, a portion of mutant glyT tRNA molecules contained N6-(delta 2-isopentenyl)-2-thiomethyl adenine adjacent to the 3' end of the anticodon (nucleotide 37). The presence or absence of this hypermodification may be a determinant in some of the biological properties of the mutant tRNA.

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Selected References

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