Skip to main content
NCBI home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1981 Dec 21;9(24):7013–7032. doi: 10.1093/nar/9.24.7013

Sequence complexity and diversity of polyadenylated RNA molecules of human normal resting, PHA-stimulated and leukemic lymphocytes.

G Torelli, S Ferrari, A Donelli, R Cadossi, S Ferrari, P Bosi, U Torelli
PMCID: PMC327658  PMID: 6174950

Abstract

The abundance and complexity of total poly(A)+RNA of human normal unstimulated lymphocytes, PHA-stimulated and leukemic lymphocytes were examined using the cDNA-poly(A)+ RNA hybridization technique. It was found that in both PHA-stimulated and leukemic lymphocytes the complexity of total poly(A)+RNA is strongly reduced in respect to normal unstimulated lymphocytes. The differences is due to a small portion, by weight, of high complexity sequences, suggesting the involvement or nuclear, not necessarily transcriptional, events. Moreover the heterologous hybridizations show that: 1) in PHA-stimulated lymphocytes there is a set of highly abundant sequences which are represented in normal and leukemic lymphocytes at a very reduced rate; 2) the sequences abundant in normal unstimulated lymphocytes are less represented in leukemic lymphocytes; the converse also is true, but at a lower extent. On the basis of available data, it is suggested that the observed changes in the abundance pattern of PHA-stimulated lymphocytes may be related mainly to a difference in the half-life of the mRNAs, while those of leukemic lymphocytes require some transcriptional or post-transcriptional nuclear event.

Full text

PDF
7013

Images in this article

7026Image
on p.7026

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Affara N. A., Robert B., Jacquet M., Buckingham M. E., Gros F. Changes in gene expression during myogenic differentiation. I. Regulation of messenger RNA sequences expressed during myotube formation. J Mol Biol. 1980 Jul 15;140(4):441–458. doi: 10.1016/0022-2836(80)90264-8. [DOI] [PubMed] [Google Scholar]
  2. Arthur C. G., Weide C. M., Vincent W. S., 3rd, Goldstein E. S. mRNA sequence diversity during early embryogenesis in Drosophila melanogaster. Exp Cell Res. 1979 Jun;121(1):87–94. doi: 10.1016/0014-4827(79)90447-6. [DOI] [PubMed] [Google Scholar]
  3. Aviv H., Leder P. Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose. Proc Natl Acad Sci U S A. 1972 Jun;69(6):1408–1412. doi: 10.1073/pnas.69.6.1408. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Axel R., Feigelson P., Schutz G. Analysis of the complexity and diversity of mRNA from chicken liver and oviduct. Cell. 1976 Feb;7(2):247–254. doi: 10.1016/0092-8674(76)90024-6. [DOI] [PubMed] [Google Scholar]
  5. Bailey J. M., Davidson N. Methylmercury as a reversible denaturing agent for agarose gel electrophoresis. Anal Biochem. 1976 Jan;70(1):75–85. doi: 10.1016/s0003-2697(76)80049-8. [DOI] [PubMed] [Google Scholar]
  6. Balmain A., Minty A. J., Birnie G. D. Frequency distribution of pre-messenger RNA sequences in polyadenylated and non-polyadenylated nuclear RNA from Friend cells. Nucleic Acids Res. 1980 Apr 11;8(7):1643–1660. doi: 10.1093/nar/8.7.1643. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Bantle J. A., Maxwell I. H., Hahn W. E. Specificity of oligo (dT)-cellulose chromatography in the isolation of polyadenylated RNA. Anal Biochem. 1976 May 7;72:413–427. doi: 10.1016/0003-2697(76)90549-2. [DOI] [PubMed] [Google Scholar]
  8. Berger S. L., Birkenmeier C. S. Metabolism of polyadenylated mRNA in growing human lymphocytes. Biochim Biophys Acta. 1979 Mar 28;562(1):80–91. doi: 10.1016/0005-2787(79)90128-x. [DOI] [PubMed] [Google Scholar]
  9. Berger S. L., Cooper H. L. Very short-lived and stable mRNAs from resting human lymphocytes. Proc Natl Acad Sci U S A. 1975 Oct;72(10):3873–3877. doi: 10.1073/pnas.72.10.3873. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Biessmann H. Concentrations of individual RNA sequences in polyadenylated nuclear and cytoplasmic RNA populations of Drosophila cells. Nucleic Acids Res. 1980 Dec 20;8(24):6099–6111. doi: 10.1093/nar/8.24.6099. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Birnie G. D., Balmain A., Jacobs H., Shott R., Wilkes P. R., Paul J. Post-transcriptional control of messenger abundance. Mol Biol Rep. 1981 May 22;7(1-3):159–162. doi: 10.1007/BF00778747. [DOI] [PubMed] [Google Scholar]
  12. Birnie G. D., MacPhail E., Young B. D., Getz M. J., Paul J. The diversity of the messenger RNA population in growing Friend cells. Cell Differ. 1974 Nov;3(4):221–232. doi: 10.1016/0045-6039(74)90005-0. [DOI] [PubMed] [Google Scholar]
  13. Bishop J. O., Morton J. G., Rosbash M., Richardson M. Three abundance classes in HeLa cell messenger RNA. Nature. 1974 Jul 19;250(463):199–204. doi: 10.1038/250199a0. [DOI] [PubMed] [Google Scholar]
  14. Bishop J. O., Rosbash M. Polynucleotide sequences in eukaryotic DNA and RNA that form ribonuclease-resistant complexes with polyuridylic acid. J Mol Biol. 1974 May 5;85(1):75–86. doi: 10.1016/0022-2836(74)90130-2. [DOI] [PubMed] [Google Scholar]
  15. Capetanaki Y. G., Alonso A. Comparison of polysomal and nuclear poly(A)-containing RNA populations from normal rat liver and Novikoff hepatoma. Nucleic Acids Res. 1980 Jul 25;8(14):3193–3214. doi: 10.1093/nar/8.14.3193. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Chikaraishi D. M., Deeb S. S., Sueoka N. Sequence complexity of nuclear RNAs in adult rat tissues. Cell. 1978 Jan;13(1):111–120. doi: 10.1016/0092-8674(78)90142-3. [DOI] [PubMed] [Google Scholar]
  17. Costantini F. D., Britten R. J., Davidson E. H. Message sequences and short repetitive sequences are interspersed in sea urchin egg poly(A)+ RNAs. Nature. 1980 Sep 11;287(5778):111–117. doi: 10.1038/287111a0. [DOI] [PubMed] [Google Scholar]
  18. Croizat B., Berthelot F., Felsani A., Gros F. Complexity of polysomal polyadenylated RNA in mouse whole brain and cortex. FEBS Lett. 1979 Jul 1;103(1):138–143. doi: 10.1016/0014-5793(79)81267-3. [DOI] [PubMed] [Google Scholar]
  19. Getz M. J., Birnie G. D., Young B. D., MacPhail E., Paul J. A kinetic estimation of base sequence complexity of nuclear poly(A)-containing RNA in mouse Friend cells. Cell. 1975 Feb;4(2):121–129. doi: 10.1016/0092-8674(75)90118-x. [DOI] [PubMed] [Google Scholar]
  20. Getz M. J., Elder P. K., Benz E. W., Jr, Stephens R. E., Moses H. L. Effect of cell proliferation on levels and diversity of poly(A)-containing mRNA. Cell. 1976 Feb;7(2):255–65. doi: 10.1016/0092-8674(76)90025-8. [DOI] [PubMed] [Google Scholar]
  21. Getz M. J., Reiman H. M., Jr, Siegal G. P., Quinlan T. J., Proper J., Elder P. K., Moses H. L. Gene expression in chemically transformed mouse embryo cells: selective enhancement of the expression of C type RNA tumor virus genes. Cell. 1977 Aug;11(4):909–921. doi: 10.1016/0092-8674(77)90302-6. [DOI] [PubMed] [Google Scholar]
  22. Grady L. J., Campbell W. P., North A. B. A comparison of several procedures for reducing RNase contamination in preparations of DNase and a particularly successful combination of methods. Anal Biochem. 1980 Jan 1;101(1):118–122. doi: 10.1016/0003-2697(80)90049-4. [DOI] [PubMed] [Google Scholar]
  23. Hereford L. M., Rosbash M. Number and distribution of polyadenylated RNA sequences in yeast. Cell. 1977 Mar;10(3):453–462. doi: 10.1016/0092-8674(77)90032-0. [DOI] [PubMed] [Google Scholar]
  24. Holland C. A., Mayrand S., Pederson T. Sequence complexity of nuclear and messenger RNA in HeLa cells. J Mol Biol. 1980 Apr 25;138(4):755–778. doi: 10.1016/0022-2836(80)90064-9. [DOI] [PubMed] [Google Scholar]
  25. Jacobs H., Birnie G. D. Post-transcriptional regulation of messenger abundance in rat liver and hepatoma. Nucleic Acids Res. 1980 Jul 25;8(14):3087–3103. doi: 10.1093/nar/8.14.3087. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Jelinek W., Evans R., Wilson M., Salditt-Georgieff M., Darnell J. E. Oligonucleotides in heterogeneous nuclear RNA: similarity of inverted repeats and RNA from repetitious DNA sites. Biochemistry. 1978 Jul 11;17(14):2776–2783. doi: 10.1021/bi00607a012. [DOI] [PubMed] [Google Scholar]
  27. Klein W. H., Murphy W., Attardi G., Britten R. J., Davidson E. H. Distribution of repetitive and nonrepetivite sequence transcripts in HeLa mRNA. Proc Natl Acad Sci U S A. 1974 May;71(5):1785–1789. doi: 10.1073/pnas.71.5.1785. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Knöchel W., Patel N. T., Holoubek V. Polysomal poly(A)+ RNA in liver of rats fed 3'-methyl-4-dimethylaminoazobenzene and in hepatoma induced by the same carcinogen. Biochim Biophys Acta. 1980;606(1):67–75. doi: 10.1016/0005-2787(80)90098-2. [DOI] [PubMed] [Google Scholar]
  29. Kuo M. T., Ried M. E., Saunders G. F. Abundancy and diversity of mRNA sequences in human leukocytes. Differentiation. 1976 Jun 4;5(2-3):175–178. doi: 10.1111/j.1432-0436.1976.tb00911.x. [DOI] [PubMed] [Google Scholar]
  30. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  31. Levy B., McCarthy B. J. Relationship between nuclear and cytoplasmic RNA in Drosophilia cells. Biochemistry. 1976 Jun 1;15(11):2415–2419. doi: 10.1021/bi00656a026. [DOI] [PubMed] [Google Scholar]
  32. Maxwell I. H., Maxwell F., Hahn W. E. Use of CH3HgOH-agarose gels for the electrophoresis of heterogeneous nuclear RNA and messenger RNA from mammalian cells. Anal Biochem. 1979 Oct 15;99(1):146–160. doi: 10.1016/0003-2697(79)90056-3. [DOI] [PubMed] [Google Scholar]
  33. Meyuhas O., Perry R. P. Relationship between size, stability and abundance of the messenger RNA of mouse L cells. Cell. 1979 Jan;16(1):139–148. doi: 10.1016/0092-8674(79)90195-8. [DOI] [PubMed] [Google Scholar]
  34. Monahan J. J., Harris S. E., Woo S. L., Robberson D. L., O'Malley B. W. The synthesis and properties of the complete complementary DNA transcript of ovalbumin mRNA. Biochemistry. 1976 Jan 13;15(1):223–233. doi: 10.1021/bi00646a034. [DOI] [PubMed] [Google Scholar]
  35. Ostrow R. S., Woods W. G., Vosika G. J., Faras A. J. Analysis of the genetic complexity and abundance classes of messenger RNA in human liver and leukemic cells. Biochim Biophys Acta. 1979 Mar 28;562(1):92–102. doi: 10.1016/0005-2787(79)90129-1. [DOI] [PubMed] [Google Scholar]
  36. Pelham H. R., Jackson R. J. An efficient mRNA-dependent translation system from reticulocyte lysates. Eur J Biochem. 1976 Aug 1;67(1):247–256. doi: 10.1111/j.1432-1033.1976.tb10656.x. [DOI] [PubMed] [Google Scholar]
  37. Reiners J. J., Jr, Busch H. Transcriptional and posttranscriptional modulation of cytoplasmic ribonucleic acids in regenerating liver and Novikoff hepatoma. Biochemistry. 1980 Mar 4;19(5):833–841. doi: 10.1021/bi00546a002. [DOI] [PubMed] [Google Scholar]
  38. Rolton H. A., Birnie G. D., Paul J. The diversity and specificity of nuclear and polysomal poly(A)+ RNA populations in normal and MSV-transformed cells. Cell Differ. 1977 Jun;6(1):25–39. doi: 10.1016/0045-6039(77)90042-2. [DOI] [PubMed] [Google Scholar]
  39. Savage M. J., Sala-Trepat J. M., Bonner J. Measurement of the complexity and diversity of poly(adenylic acid) containing messenger RNA from rat liver. Biochemistry. 1978 Feb 7;17(3):462–467. doi: 10.1021/bi00596a014. [DOI] [PubMed] [Google Scholar]
  40. Tashima M., Calabretta B., Torelli G., Scofield M., Maizel A., Saunders G. F. Presence of a highly repetitive and widely dispersed DNA sequence in the human genome. Proc Natl Acad Sci U S A. 1981 Mar;78(3):1508–1512. doi: 10.1073/pnas.78.3.1508. [DOI] [PMC free article] [PubMed] [Google Scholar]
  41. Torelli G., Cadossi R., Ferrari S., Narni F., Ferrari S., Montagnani G., Torelli U., Bosi P. Reassociation kinetics of the DNA of human acute leukemia cells. Biochim Biophys Acta. 1979 Feb 27;561(2):301–311. doi: 10.1016/0005-2787(79)90139-4. [DOI] [PubMed] [Google Scholar]
  42. Wang D., Moore S. Preparation of protease-free and ribonuclease-free pancreatic deoxyribonuclease. J Biol Chem. 1978 Oct 25;253(20):7216–7219. [PubMed] [Google Scholar]
  43. Williams J. G., Hoffman R., Penman S. The extensive homology between mRNA sequences of normal and SV40-transformed human fibroblasts. Cell. 1977 Aug;11(4):901–907. doi: 10.1016/0092-8674(77)90301-4. [DOI] [PubMed] [Google Scholar]
  44. Williams J. G., Penman S. The messenger RNA sequences in growing and resting mouse fibroblasts. Cell. 1975 Oct;6(2):197–206. doi: 10.1016/0092-8674(75)90010-0. [DOI] [PubMed] [Google Scholar]
  45. Woods D., Crampton J., Clarke B., Williamson R. The construction of a recombinant cDNA library representative of the poly(A)+ mRNA population from normal human lymphocytes. Nucleic Acids Res. 1980 Nov 25;8(22):5157–5168. doi: 10.1093/nar/8.22.5157. [DOI] [PMC free article] [PubMed] [Google Scholar]
  46. Young B. D., Birnie G. D. Complexity and specificity of polysomal poly(A+) RNA in mouse tissues. Biochemistry. 1976 Jun 29;15(13):2823–2829. doi: 10.1021/bi00658a019. [DOI] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES