Skip to main content
NCBI home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1979 Jan;6(1):27–39. doi: 10.1093/nar/6.1.27

The primary transcript of the ribosomal repeating unit in yeast.

J Klootwijk, P de Jonge, R J Planta
PMCID: PMC327671  PMID: 106368

Abstract

Endgroup analysis of 37S ribosomal precursor RNA from Saccharomyces carlsbergensis has revealed that the major 5' endgroup is ppA-Up, with a molar yield of 0.8. This shows that most, if not all, 37S RNA molecules have preserved a transcriptional initiation sequence. Analysis of the 3' terminus of 37S RNA has shown the presence of a uridine rich oligonucleotide, tentatively identified as U6-8-A-NOH. This long stretch of uridines at the 3' end of 37S RNA may represent a transcriptional termination site. The two sets of data on the terminal sequences suggest that 37S ribosomal precursor RNA, if not already spliced, is a primary transcription product. Since the 3' terminus of 26S rRNA, U-U-U-G-UOH., appears to be clearly different from the 3' end of 37S RNA, we conclude that 37S ribosomal precursor RNA contains additional nucleotides 3'-distal to the 26S rRNA sequence.

Full text

PDF
27

Images in this article

31Image
on p.31
32Image
on p.32
33Image
on p.33
35Image
on p.35

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Batts-Young B., Lodish H. F. Triphosphate residues at the 5' ends of rRNA precursor and 5S RNA from Dictyostelium discoideum. Proc Natl Acad Sci U S A. 1978 Feb;75(2):740–744. doi: 10.1073/pnas.75.2.740. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Brand R. C., Klootwijk J., Van Steenbergen T. J., De Kok A. J., Planta R. J. Secondary methylation of yeast ribosomal precursor RNA. Eur J Biochem. 1977 May 2;75(1):311–318. doi: 10.1111/j.1432-1033.1977.tb11531.x. [DOI] [PubMed] [Google Scholar]
  3. Brand R. C., Planta R. J. The molecular weights of yeast ribosomal precursor RNAs. Mol Biol Rep. 1975 Dec;2(4):321–325. doi: 10.1007/BF00357019. [DOI] [PubMed] [Google Scholar]
  4. Brownlee G. G., Sanger F. Chromatography of 32P-labelled oligonucleotides on thin layers of DEAE-cellulose. Eur J Biochem. 1969 Dec;11(2):395–399. doi: 10.1111/j.1432-1033.1969.tb00786.x. [DOI] [PubMed] [Google Scholar]
  5. Dahlberg J. E. Terminal sequences of bacteriophage RNAs. Nature. 1968 Nov 9;220(5167):548–552. doi: 10.1038/220548a0. [DOI] [PubMed] [Google Scholar]
  6. De Jonge P., Klootwijk J., Planta R. J. Sequence of the 3'-terminal 21 nucleotides of yeast 17S ribosomal RNA. Nucleic Acids Res. 1977 Oct;4(10):3655–3663. doi: 10.1093/nar/4.10.3655. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. De Jonge P., Klootwijk J., Planta R. J. Terminal nucleotide sequences of 17-S ribosomal RNA and its immediate precursor 18-S RNA in yeast. Eur J Biochem. 1977 Jan;72(2):361–369. doi: 10.1111/j.1432-1033.1977.tb11260.x. [DOI] [PubMed] [Google Scholar]
  8. Goodman H. M., Olson M. V., Hall B. D. Nucleotide sequence of a mutant eukaryotic gene: the yeast tyrosine-inserting ochre suppressor SUP4-o. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5453–5457. doi: 10.1073/pnas.74.12.5453. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Jordan B. R., Galling G., Jourdan R. Sequence and conformation of 5 S RNA from Chlorella cytoplasmic ribosomes: comparison with other 5 S RNA molecules. J Mol Biol. 1974 Aug 5;87(2):205–225. doi: 10.1016/0022-2836(74)90144-2. [DOI] [PubMed] [Google Scholar]
  10. Maxam A. M., Tizard R., Skryabin K. G., Gilbert W. Promotor region for yeast 5S ribosomal RNA. Nature. 1977 Jun 16;267(5612):643–645. doi: 10.1038/267643a0. [DOI] [PubMed] [Google Scholar]
  11. Meyerink J. H., Retèl J., Raué H. A., Planta R. J. Genetic organization of the ribosomal transcription units of the yeast Saccharomyces carlsbergensis. Nucleic Acids Res. 1978 Aug;5(8):2801–2808. doi: 10.1093/nar/5.8.2801. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Min Jou W., Fiers W. Sequence determination of Gp-rich oligonucleotides by means of the Kethoxal modification. FEBS Lett. 1976 Jul 1;66(1):77–81. doi: 10.1016/0014-5793(76)80589-3. [DOI] [PubMed] [Google Scholar]
  13. Philippsen P., Thomas M., Kramer R. A., Davis R. W. Unique arrangement of coding sequences for 5 S, 5.8 S, 18 S and 25 S ribosomal RNA in Saccharomyces cerevisiae as determined by R-loop and hybridization analysis. J Mol Biol. 1978 Aug 15;123(3):387–404. doi: 10.1016/0022-2836(78)90086-4. [DOI] [PubMed] [Google Scholar]
  14. Planta R. J., Retèl J., Klootwijk J., Meyerink J. H., De Jonge P., Van Keulen H., Brand R. C. Synthesis and processing of ribosomal ribonucleic acid in eukaryotes. Biochem Soc Trans. 1977;5(2):462–466. doi: 10.1042/bst0050462. [DOI] [PubMed] [Google Scholar]
  15. Reeder R. H., Sollner-Webb B., Wahn H. L. Sites of transcription initiation in vivo on Xenopus laevis ribosomal DNA. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5402–5406. doi: 10.1073/pnas.74.12.5402. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Retèl J., Planta R. J. On the mechanism of the biosynthesis of ribosomal RNA in yeast. Biochim Biophys Acta. 1970 Dec 14;224(2):458–469. doi: 10.1016/0005-2787(70)90578-2. [DOI] [PubMed] [Google Scholar]
  17. Retèl J., Planta R. J. Ribosomal precursor RNA in Saccharomyces carlsbergensis. Eur J Biochem. 1967 Dec;3(2):248–258. doi: 10.1111/j.1432-1033.1967.tb19524.x. [DOI] [PubMed] [Google Scholar]
  18. Rosenberg M. Isolation and sequence determination of the 3'-terminal regions of isotopically labelled RNA molecules. Nucleic Acids Res. 1974 May;1(5):653–671. doi: 10.1093/nar/1.5.653. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Rubin G. M. The nucleotide sequence of Saccharomyces cerevisiae 5.8 S ribosomal ribonucleic acid. J Biol Chem. 1973 Jun 10;248(11):3860–3875. [PubMed] [Google Scholar]
  20. Sanger F., Brownlee G. G., Barrell B. G. A two-dimensional fractionation procedure for radioactive nucleotides. J Mol Biol. 1965 Sep;13(2):373–398. doi: 10.1016/s0022-2836(65)80104-8. [DOI] [PubMed] [Google Scholar]
  21. Shine J., Hunt J. A., Dalgarno L. Studies on the 3'-terminal sequences of the large ribosomal ribonucleic acid of different eukaryotes and those associated with "hidden" breaks in heart-dissociable insects 26S ribonucleic acid. Biochem J. 1974 Sep;141(3):617–625. doi: 10.1042/bj1410617. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Trapman J., Planta R. J. Detailed analysis of the ribosomal RNA synthesis in yeast. Biochim Biophys Acta. 1975 Dec 4;414(2):115–125. doi: 10.1016/0005-2787(75)90214-2. [DOI] [PubMed] [Google Scholar]
  23. Trapman J., Retèl J., Planta R. J. Ribosomal precursor particles from yeast. Exp Cell Res. 1975 Jan;90(1):95–104. doi: 10.1016/0014-4827(75)90361-4. [DOI] [PubMed] [Google Scholar]
  24. Udem S. A., Warner J. R. Ribosomal RNA synthesis in Saccharomyces cerevisiae. J Mol Biol. 1972 Mar 28;65(2):227–242. doi: 10.1016/0022-2836(72)90279-3. [DOI] [PubMed] [Google Scholar]
  25. Valenzuela P., Bell G. I., Masiarz F. R., DeGennaro L. J., Rutter W. J. Nucleotide sequence of the yeast 5S ribosomal RNA gene and adjacent putative control regions. Nature. 1977 Jun 16;267(5612):641–643. doi: 10.1038/267641a0. [DOI] [PubMed] [Google Scholar]
  26. Valenzuela P., Venegas A., Weinberg F., Bishop R., Rutter W. J. Structure of yeast phenylalanine-tRNA genes: an intervening DNA segment within the region coding for the tRNA. Proc Natl Acad Sci U S A. 1978 Jan;75(1):190–194. doi: 10.1073/pnas.75.1.190. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Van Keulen H., Planta R. J., Retèl J. Structure and transcription specificity of yeast RNA polymerase A. Biochim Biophys Acta. 1975 Jun 16;395(2):179–190. doi: 10.1016/0005-2787(75)90157-4. [DOI] [PubMed] [Google Scholar]
  28. Van Keulen H., Retèl J. Transcription specificity of yeast RNA polymerase A. Highly specific transcription in vitro of the homologous ribosomal transcription units. Eur J Biochem. 1977 Oct 3;79(2):579–588. doi: 10.1111/j.1432-1033.1977.tb11842.x. [DOI] [PubMed] [Google Scholar]
  29. van den Bos R. C., Klootwijk J., Planta R. J. Structural comparison of 17 S ribosomal RNA of yeast and its immediate precursor, 18 S RNA. FEBS Lett. 1972 Jul 15;24(1):93–97. doi: 10.1016/0014-5793(72)80834-2. [DOI] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES