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. 1979 Jan;6(1):71–80. doi: 10.1093/nar/6.1.71

Origin and direction of replication of the bacteriocinogenic plasmid Clo DF13.

A R Stuitje, E Veltkamp, P J Weijers, H J Nijkamp
PMCID: PMC327674  PMID: 370788

Abstract

Cairn's type replicative intermediates of both the wildtype Clo DF13 plasmid and the copy mutant CLO DF13 cop3 were isolated by dye-buoyant density centrifugation. Replicative intermediates were linearized at the HpaI or Sa1I cleavage site, and examined with the electron-microscope. The data show that replication of both the Clo DF13 wild type plasmid and the Clo DF13 cop3 plasmid, initiates at about 2.8% on the physical map. Replication proceeds unindirectionally and counterclockwise on this map.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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