Abstract
Cairn's type replicative intermediates of both the wildtype Clo DF13 plasmid and the copy mutant CLO DF13 cop3 were isolated by dye-buoyant density centrifugation. Replicative intermediates were linearized at the HpaI or Sa1I cleavage site, and examined with the electron-microscope. The data show that replication of both the Clo DF13 wild type plasmid and the Clo DF13 cop3 plasmid, initiates at about 2.8% on the physical map. Replication proceeds unindirectionally and counterclockwise on this map.
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Selected References
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- Adler H. I., Fisher W. D., Cohen A., Hardigree A. A. MINIATURE escherichia coli CELLS DEFICIENT IN DNA. Proc Natl Acad Sci U S A. 1967 Feb;57(2):321–326. doi: 10.1073/pnas.57.2.321. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Andreoli P. M., Brandsma R. A., Veltkamp E., Nijkamp H. J. Isolation and characterization of a Clo DF13::Tn901 plasmid mutant with thermosensitive control of DNA replication. J Bacteriol. 1978 Aug;135(2):612–621. doi: 10.1128/jb.135.2.612-621.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Andreoli P. M., Brandsma R. A., Veltkamp E., Nijkamp H. J. Isolation and characterization of a Clo DF13::Tn901 plasmid mutant with thermosensitive control of DNA replication. J Bacteriol. 1978 Aug;135(2):612–621. doi: 10.1128/jb.135.2.612-621.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Clewell D. B. Nature of Col E 1 plasmid replication in Escherichia coli in the presence of the chloramphenicol. J Bacteriol. 1972 May;110(2):667–676. doi: 10.1128/jb.110.2.667-676.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Crosa J. H., Luttropp L. K., Heffron F., Falkow S. Two replication initiation sites on R-plasmid DNA. Mol Gen Genet. 1975 Sep 15;140(1):39–50. doi: 10.1007/BF00268987. [DOI] [PubMed] [Google Scholar]
- Inselburg J. Replication of colicin E1 plasmid DNA in minicells from a unique replication initiation site. Proc Natl Acad Sci U S A. 1974 Jun;71(6):2256–2259. doi: 10.1073/pnas.71.6.2256. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kingsbury D. T., Helinski D. R. DNA polymerase as a requirement for the maintenance of the bacterial plasmid colicinogenic factor E1. Biochem Biophys Res Commun. 1970 Dec 24;41(6):1538–1544. doi: 10.1016/0006-291x(70)90562-0. [DOI] [PubMed] [Google Scholar]
- Kool A. J., Pranger M., Nijkamp H. J. Proteins synthesized by a non-induced bacteriocinogenic factor in minicells of Escherichia coli. Mol Gen Genet. 1972;115(4):314–323. doi: 10.1007/BF00333170. [DOI] [PubMed] [Google Scholar]
- Stuitje A. R., Veltkamp E., van den Elzen P. J., Nijkamp H. J. In vitro construction of deletion mutants of the bacteriocinogenic plasmid Clo DF13. Nucleic Acids Res. 1978 Jun;5(6):1801–1820. doi: 10.1093/nar/5.6.1801. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tomizawa J. I., Ohmori H., Bird R. E. Origin of replication of colicin E1 plasmid DNA. Proc Natl Acad Sci U S A. 1977 May;74(5):1865–1869. doi: 10.1073/pnas.74.5.1865. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tomizawa J., Sakakibara Y., Kakefuda T. Replication of colicin E1 plasmid DNA in cell extracts. Origin and direction of replication. Proc Natl Acad Sci U S A. 1974 Jun;71(6):2260–2264. doi: 10.1073/pnas.71.6.2260. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Veltkamp E., Blankevoort R., Nijkamp H. J. Binding of bacteriocin Clo DF13 to Clo DF13 plasmid deoxyribonucleic acid in vivo and in vitro. Antimicrob Agents Chemother. 1975 Sep;8(3):356–365. doi: 10.1128/aac.8.3.356. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Veltkamp E., Nijkamp H. J. Characterization of a replication mutant of the bacteriocinogenic plasmid Clo DF13. Biochim Biophys Acta. 1976 Mar 17;425(3):356–367. doi: 10.1016/0005-2787(76)90263-x. [DOI] [PubMed] [Google Scholar]
- Veltkamp E., Nijkamp H. J. Replication of the bacteriocinogenic plasmid Clo DF13 in thermosensitive Escherichia coli mutants defective in initiation or elongation of deoxyribonucleic acid replication. J Bacteriol. 1974 Dec;120(3):1227–1237. doi: 10.1128/jb.120.3.1227-1237.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- van Embden J. D., Veltkamp E., Stuitje T., Andreoli P. M., Nijkamp H. J. Integration of a transposable DNA sequence which mediates ampicillin resistance into Clo DF13 plasmid DNA: determination of the site and orientation of TnA insertions. Plasmid. 1978 Feb;1(2):204–217. doi: 10.1016/0147-619x(78)90039-2. [DOI] [PubMed] [Google Scholar]