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. 1979 Jan;6(1):219–230. doi: 10.1093/nar/6.1.219

Analysis of the attachment of replicating DNA to a nuclear matrix in mammalian interphase nuclei.

P A Dijkwel, L H Mullenders, F Wanka
PMCID: PMC327684  PMID: 424290

Abstract

The attachment of replicating DNA to a rapidly sedimenting nuclear structure was investigated by digestion with various nucleases. When DNA was gradually removed by DNase I, pulse label incorporated during either 1 min or during 1 hour in the presence of arabinosylcytosine, remained preferentially attached to the nuclear structure. Single strand specific digestion by nuclease S1 or staphylococcal nuclease at low concentrations caused a release of about 30% of the pulse label, without significantly affecting the attachment of randomly labelled DNA. The released material had a low sedimentation coefficient and contained most of the Okasaki fragments. The remaining pulse label was less accessible to further digestion by double strand specific nuclease activity than the bulk DNA. The results suggest that an attachment of the replication fork to the nuclear structure occurs at sites behind but close to the branch point.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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