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. 1979 Jan;6(1):231–246. doi: 10.1093/nar/6.1.231

Alkaline deoxyribonucleases released from Neurospora crassa mycelia: two activities not released by mutants with multiple sensitivities to mutagens.

M J Fraser
PMCID: PMC327685  PMID: 154656

Abstract

Three major alkaline deoxyribonuclease (DNase) activities have been identified in sorbose-containing liquid culture medium in which wild-type Neurosporacrassa were grown: DNase A, a Ca++dependent endonuclease of molecular weight 65,000 daltons which has no specificity for single- or double-stranded DNA (ss-DNA or ds-DNA) and no activity with RNA; DNase B, a Mg++-dependent single-strand specific exonuclease of molecular weight 78,000 daltons active with both ss-DNA and RNA; DNase C, a divalent metal ion-dependent endo-exonuclease of molecular weight 65,000 having single-strand specific endonuclease activity with ss-DNA and RNA and exonuclease activity with ds-DNA. Three mutants which were shown previously to have wide spectra of sensitivities to mutagens, and which exhibited reduced release of DNase activity on sorbose-containing agar test plates (the Nuh phenotype), were deficient relative to the wild-type in the release of these major alkaline DNases into the liquid culture medium. The uvs-3 mutant released only small amounts of DNase A and DNase C; nuh-4 did not release detectable DNase C and released only a very low level of DNase B; uvs-6 released only a low level of DNase A. A nuh mutant (nuh-3) which is not mutagen sensitive relative to the wild-type released low levels of DNase B. On the other hand, an ultraviolet light-sensitive mutant (nuc-2) which does not have the Nuh phenotype was normal in the release of these DNases.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Clark A. J. Recombination deficient mutants of E. coli and other bacteria. Annu Rev Genet. 1973;7:67–86. doi: 10.1146/annurev.ge.07.120173.000435. [DOI] [PubMed] [Google Scholar]
  2. Fraser M. J., Tjeerde R., Matsumoto K. A second form of the single-strand specific endonuclease of Neurospora crassa which is associated with a double-strand exonuclease. Can J Biochem. 1976 Nov;54(11):971–980. doi: 10.1139/o76-140. [DOI] [PubMed] [Google Scholar]
  3. HOLLIDAY R. THE INDUCTION OF MITOTIC RECOMBINATION BY MITOMYCIN C IN USTILAGO AND SACCHAROMYCES. Genetics. 1964 Sep;50:323–335. doi: 10.1093/genetics/50.3.323. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hasunuma K. Repressible extracellular nucleases in Neurospora crassa. Biochim Biophys Acta. 1973 Sep 7;319(3):288–293. doi: 10.1016/0005-2787(73)90168-8. [DOI] [PubMed] [Google Scholar]
  5. Holloman W. K., Holliday R. Studies on a nuclease from Ustilago maydis. I. Purification, properties, and implication in recombination of the enzyme. J Biol Chem. 1973 Dec 10;248(23):8107–8113. [PubMed] [Google Scholar]
  6. Ishikawa T., Toh-E A., Uno I., Hasunuma K. Isolation and characterization of nuclease mutants in Neurospora crassa. Genetics. 1969 Sep;63(1):75–92. doi: 10.1093/genetics/63.1.75. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kwong S., Fraser M. J. Neurospora endoexonuclease and its inactive (precursor?) form. Can J Biochem. 1978 Jun;56(6):370–377. doi: 10.1139/o78-059. [DOI] [PubMed] [Google Scholar]
  8. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  9. MARTIN R. G., AMES B. N. A method for determining the sedimentation behavior of enzymes: application to protein mixtures. J Biol Chem. 1961 May;236:1372–1379. [PubMed] [Google Scholar]
  10. Mills C., Fraser M. J. Different chromatographic forms of Neurospora crassa nucleases specific for single-stranded nucleic acids. Can J Biochem. 1973 Jun;51(6):888–895. doi: 10.1139/o73-110. [DOI] [PubMed] [Google Scholar]
  11. Rabin E. Z., Preiss B., Fraser M. J. A nuclease from Neurospora crassa conidia specific for single-stranded nucleic acids. Prep Biochem. 1971;1(4):283–307. doi: 10.1080/00327487108081946. [DOI] [PubMed] [Google Scholar]
  12. Rabin E. Z., Tenenhouse H., Fraser M. J. An exonuclease of Neurospora crassa specific for single-stranded nucleic acids. Biochim Biophys Acta. 1972 Jan 18;259(1):50–68. doi: 10.1016/0005-2787(72)90473-x. [DOI] [PubMed] [Google Scholar]
  13. Schroeder A. L. Ultraviolet-sensitive mutants of Neurospora. I. Genetic basis and effect on recombination. Mol Gen Genet. 1970;107(4):291–304. doi: 10.1007/BF00441192. [DOI] [PubMed] [Google Scholar]
  14. Schroeder A. Genetic control of radiation sensitivity and DNA repair in Neurospora. Basic Life Sci. 1975;5B:567–576. doi: 10.1007/978-1-4684-2898-8_22. [DOI] [PubMed] [Google Scholar]
  15. Tenenhouse H., Fraser M. J. The ribonuclease activities of the single-strand-specific nucleases of Neurospora crassa. Can J Biochem. 1973 May;51(5):569–580. doi: 10.1139/o73-071. [DOI] [PubMed] [Google Scholar]

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