Abstract
A Y-base derivative was isolated from rat liver tRNAPhe and its structure was assigned to be alpha-(carboxyamino)-beta-hydroxy-4,9-dihydro-4,6-dimethyl-9-oxo-1H-imidazol[1,2-a]purine-7-butyric acid dimethyl ester (hydroxy-Y), based on the results of mass spectrometry and chemical degradation. This modified base seems to be the major fluorescent component of rat liver tRNAPhe; the peroxy-Y base previously isolated from rat liver tRNAPhe and characterized by Nakanishi and his coworkers (1,2) was not present in our preparation.
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Selected References
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- Blobstein S. H., Grunberger D., Weinstein I. B., Nakanishi K. Isolation and structure determination of the fluorescent base from bovine liver phenylalanine transfer ribonucleic acid. Biochemistry. 1973 Jan 16;12(2):188–193. doi: 10.1021/bi00726a002. [DOI] [PubMed] [Google Scholar]
- Feinberg A. M., Nakanishi K., Barciszewski J., Rafalski A. J., Augustyniak H., Wiewiórowski M. Isolation and characterization of peroxy-Y base from phenylalanine transfer ribonucleic acid of the plant, Lupinus luteus. J Am Chem Soc. 1974 Dec 11;96(25):7797–7780. doi: 10.1021/ja00832a029. [DOI] [PubMed] [Google Scholar]
- Kuchino Y., Borek E. Tumour-specific phenylalanine tRNA contains two supernumerary methylated bases. Nature. 1978 Jan 12;271(5641):126–129. doi: 10.1038/271126a0. [DOI] [PubMed] [Google Scholar]
- McCloskey J. A., Lawson A. M., Tsuboyama K., Krueger P. M., Stillwell R. N. Mass spectrometry of nucleic acid components. Trimethylsilyl derivatives of nucleotides, nucleosides, and bases. J Am Chem Soc. 1968 Jul 17;90(15):4182–4184. doi: 10.1021/ja01017a062. [DOI] [PubMed] [Google Scholar]
- Nakanishi K., Blobstein S., Funamizu M., Furutachi N., Van Lear G., Grunberger D., Lanks K. W., Weinstein I. B. Structure of the "peroxy-Y base" from liver tRNA Phe . Nat New Biol. 1971 Nov 24;234(47):107–109. doi: 10.1038/newbio234107b0. [DOI] [PubMed] [Google Scholar]
- Nakanishi K., Furutachi N., Funamizu M., Grunberger D., Weinstein I. B. Structure of the fluorescent Y base from yeast phenylalanine transfer ribonucleic acid. J Am Chem Soc. 1970 Dec 30;92(26):7617–7619. doi: 10.1021/ja00729a035. [DOI] [PubMed] [Google Scholar]
- Thiebe R., Zachau H. G., Baczynskyj L., Biemann K., Sonnenbichler J. Study on the properties and structure of the modified base Y+ of yeast tRNA Phe . Biochim Biophys Acta. 1971 Jun 30;240(2):163–169. doi: 10.1016/0005-2787(71)90653-8. [DOI] [PubMed] [Google Scholar]
- Yoshikami D., Keller E. B. Chemical modification of the fluorescent base in phenylalanine transfer ribonucleic acid. Biochemistry. 1971 Jul 20;10(15):2969–2976. doi: 10.1021/bi00791a027. [DOI] [PubMed] [Google Scholar]