Skip to main content
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1979 Apr;6(4):1417–1431. doi: 10.1093/nar/6.4.1417

DNA replication in Physarum polycephalum: electron microscopic and autoradiographic analysis of replicating DNA from defined stages of the S-period.

S Funderud, R Andreassen, F Haugli
PMCID: PMC327780  PMID: 450701

Abstract

Electron microscopic and autoradiographic analysis of replicating DNA from Physarum showed that replication occurs at a rate of 0.4 micron/min/per replicon and that replicons of size 10--15 mu occur in temporal clusters with an average of about 4 replicons per cluster. These results are compared with previous hydrodynamic measurements and with those obtained in other organisms.

Full text

PDF
1417

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Blumenthal A. B., Kriegstein H. J., Hogness D. S. The units of DNA replication in Drosophila melanogaster chromosomes. Cold Spring Harb Symp Quant Biol. 1974;38:205–223. doi: 10.1101/sqb.1974.038.01.024. [DOI] [PubMed] [Google Scholar]
  2. Edenberg H. J., Huberman J. A. Eukaryotic chromosome replication. Annu Rev Genet. 1975;9:245–284. doi: 10.1146/annurev.ge.09.120175.001333. [DOI] [PubMed] [Google Scholar]
  3. Funderud S., Andreassen R., Haugli F. DNA replication in Physarum polycephalum: UV photolysis of maturing 5-bromo-deoxyuridine substituted DNA. Nucleic Acids Res. 1978 Sep;5(9):3303–3313. doi: 10.1093/nar/5.9.3303. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Funderud S., Andreassen R., Haugli F. DNA replication in Physarum polycephalum: bidirectional replication of DNA within replicons. Nucleic Acids Res. 1978 Mar;5(3):713–721. doi: 10.1093/nar/5.3.713. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Funderud S., Haugli F. DNA replication in Physarum polycephalum: characterization of DNA replication products made in vivo in the presence of cycloheximide in strains sensitive and resistant to cycloheximide. Nucleic Acids Res. 1977 Feb;4(2):405–413. doi: 10.1093/nar/4.2.405. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Funderud S., Haugli F. DNA replication in Physarum polycephalum: characterization of replication products in vivo. Nucleic Acids Res. 1975 Aug;2(8):1381–1390. doi: 10.1093/nar/2.8.1381. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Funderud S., Haugli F. DNA replication in Physarum polycephalum: characterization of replication products made in isolated nuclei. Biochem Biophys Res Commun. 1977 Feb 7;74(3):941–948. doi: 10.1016/0006-291x(77)91609-6. [DOI] [PubMed] [Google Scholar]
  8. Hand R. Regulation of DNA replication on subchromosomal units of mammalian cells. J Cell Biol. 1975 Jan;64(1):89–97. doi: 10.1083/jcb.64.1.89. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hand R., Tamm I. Initiation of DNA replication in mammalian cells and its inhibition by reovirus infection. J Mol Biol. 1974 Jan 15;82(2):175–183. doi: 10.1016/0022-2836(74)90339-8. [DOI] [PubMed] [Google Scholar]
  10. Hori T., Lark K. G. Effect of puromycin on DNA replication in Chinese hamster cells. J Mol Biol. 1973 Jul 5;77(3):391–404. doi: 10.1016/0022-2836(73)90446-4. [DOI] [PubMed] [Google Scholar]
  11. Housman D., Huberman J. A. Changes in the rate of DNA replication fork movement during S phase in mammalian cells. J Mol Biol. 1975 May 15;94(2):173–181. doi: 10.1016/0022-2836(75)90076-5. [DOI] [PubMed] [Google Scholar]
  12. Huberman J. A., Riggs A. D. On the mechanism of DNA replication in mammalian chromosomes. J Mol Biol. 1968 Mar 14;32(2):327–341. doi: 10.1016/0022-2836(68)90013-2. [DOI] [PubMed] [Google Scholar]
  13. Kowalski J., Cheevers W. P. Synthesis of high molecular weight DNA strands during S phase. J Mol Biol. 1976 Jul 5;104(3):603–615. doi: 10.1016/0022-2836(76)90123-6. [DOI] [PubMed] [Google Scholar]
  14. Mohberg J., Rusch H. P. Isolation and DNA content of nuclei of Physarum polycephalum. Exp Cell Res. 1971 Jun;66(2):305–316. doi: 10.1016/0014-4827(71)90682-3. [DOI] [PubMed] [Google Scholar]
  15. Newlon C. S., Petes T. D., Hereford L. M., Fangman W. L. Replication of yeast chromosomal DNA. Nature. 1974 Jan 4;247(5435):32–35. doi: 10.1038/247032a0. [DOI] [PubMed] [Google Scholar]
  16. Planck S. R., Mueller G. C. Deoxyribonucleic acid chain growth and organization of replicating units in HeLa cells. Biochemistry. 1977 May 3;16(9):1808–1813. doi: 10.1021/bi00628a007. [DOI] [PubMed] [Google Scholar]
  17. Stimac E., Housman D., Huberman J. A. Effects of inhibition of protein synthesis on DNA replication in cultured mammalian cells. J Mol Biol. 1977 Sep 25;115(3):485–511. doi: 10.1016/0022-2836(77)90167-x. [DOI] [PubMed] [Google Scholar]
  18. Zimm B. H., Schumaker V. N., Zimm C. B. Anomalies in sedimentation. V. Chains at high fields, practical consequences. Biophys Chem. 1976 Jul;5(1-2):265–270. doi: 10.1016/0301-4622(76)80039-7. [DOI] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES