Abstract
In some preparations of DNA dependent RNA polymerase a new enzymatic activity has been found which catalyzes the condensation of two pyrophosphate molecules, liberated in the process of RNA synthesis, to one molecule of orthophosphate and one molecule of Mg (or Mn) - chelate complex with trimetaphosphate. This activity can also cooperate with DNA-polymerase, on condition that both enzymes originate from the same cells. These results point to two general conclusions. First, energy is conserved in the overall process of nucleic acid synthesis and turnover, so that the process does not require an energy influx from the cell's general resources. Second, the synthesis of nucleic acids is catalyzed by a complex enzyme system which contains at least two separate enzymes, one responsible for nucleic acid polymerization and the other for energy conservation via pyrophosphate condensation.
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- Biron K. K., Morrongiello M. P., Rasková J., Raska K., Jr Adenovirus type 12 tumor antigen. I. Separation from DNA polymerase alpha and immunoprecipitation of tumor-antigen polypeptides. Virology. 1978 Apr;85(2):464–474. doi: 10.1016/0042-6822(78)90453-1. [DOI] [PubMed] [Google Scholar]
- Burgess R. R., Jendrisak J. J. A procedure for the rapid, large-scall purification of Escherichia coli DNA-dependent RNA polymerase involving Polymin P precipitation and DNA-cellulose chromatography. Biochemistry. 1975 Oct 21;14(21):4634–4638. doi: 10.1021/bi00692a011. [DOI] [PubMed] [Google Scholar]
- Goldfarb A., Seaman E., Daniel V. In vitro transcription and isolation of a polycistronic RNA product of the T4 tRNA operon. Nature. 1978 Jun 15;273(5663):562–564. doi: 10.1038/273562a0. [DOI] [PubMed] [Google Scholar]
- KORNBERG A., KORNBERG S. R., SIMMS E. S. Metaphosphate synthesis by an enzyme from Escherichia coli. Biochim Biophys Acta. 1956 Apr;20(1):215–227. doi: 10.1016/0006-3002(56)90280-3. [DOI] [PubMed] [Google Scholar]
- KORNBERG S. R. Adenosine triphosphate synthesis from polyphosphate by an enzyme from Escherichia coli. Biochim Biophys Acta. 1957 Nov;26(2):294–300. doi: 10.1016/0006-3002(57)90008-2. [DOI] [PubMed] [Google Scholar]
- Levy S., Aviv H. Quantitation of labeled globin messenger RNA by hybridization with excess complementary DNA covalently bound to cellulose. Biochemistry. 1976 May 4;15(9):1844–1847. doi: 10.1021/bi00654a009. [DOI] [PubMed] [Google Scholar]
- Peller L. In vitro RNA synthesis should be coupled to pyrophosphate hydrolysis. Biochem Biophys Res Commun. 1975 Apr 21;63(4):912–916. doi: 10.1016/0006-291x(75)90655-5. [DOI] [PubMed] [Google Scholar]
- Salser W., Janin J., Levinthal C. Measurement of the unstable RNA in exponentially growing cultures of Bacillus subtilis and Escherichia coli. J Mol Biol. 1968 Jan 28;31(2):237–266. doi: 10.1016/0022-2836(68)90442-7. [DOI] [PubMed] [Google Scholar]
- Thompson S. T., Cass K. H., Stellwagen E. Blue dextran-sepharose: an affinity column for the dinucleotide fold in proteins. Proc Natl Acad Sci U S A. 1975 Feb;72(2):669–672. doi: 10.1073/pnas.72.2.669. [DOI] [PMC free article] [PubMed] [Google Scholar]