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. 1979;6(5):1805–1816. doi: 10.1093/nar/6.5.1805

Nucleosome cores reconstituted from poly (dA-dT) and the octamer of histones.

D Rhodes
PMCID: PMC327812  PMID: 450714

Abstract

In this paper we describe a detailed investigation of the reconstitution of nucleosome cores from poly (dA-dT) and the octamer of histones. We also attempted the reconstitution from the copolymers poly dA.poly dT, poly dG.poly dC and poly (dG-dC). The repeat of the reconstituted chromatin fibre is discussed. The micrococcal nuclease released poly (dA-dT) core particle is found to contain a considerably narrower DNA size distribution that of the native random DNA nucleosome core (12). In addition we have succeeded in obtaining small crystals of the poly (dA-dT) nucleosome core. The DNAase I digestion pattern of the poly (dA-dT) containing nucleosome core is presented. The periodicity of DNAase I cutting sites is found to be about 10.5 bases and is similar to that of the native nucleosome core (12, 13).

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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