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. 1979;6(6):2193–2208. doi: 10.1093/nar/6.6.2193

Changes in nuclear and polysomal polyadenylated RNA sequences during rat-liver regeneration.

P R Wilkes, G D Birnie, J Paul
PMCID: PMC327845  PMID: 461186

Abstract

Nuclear and polysomal polyadenylated RNA populations of normal and 16 hour regenerating rat liver have been compared by mRNA-cDNA hybridisations and by unique DNA saturation experiments. It was found that nuclear polyadenylated RNA hybridises to 6.8% of unique DNA in both normal and 16 hour regenerating rat liver. However, cross-hybridisation experiments using cDNA have shown that 10-15% by weight of nuclear polyadenylated RNA sequences are specific to 16 hour regenerating rat-liver. Since both unique DNA and cDNA hybridisation have shown that normal and 16 hour regenerating rat-liver polysomal polyadenylated RNA populations are qualitatively very similar sequences specific to 16 hour regenerating rat-liver nuclear polyadenylated RNA are nucleus confined. Polysomal RNA sequences which were abundant in normal rat-liver have become less abundant in regenerating rat liver.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BRAUN G. A., MARSH J. B., DRABKIN D. L. Synthesis of plasma albumin and tissue proteins in regenerating liver. Metabolism. 1962 Sep;11:957–966. [PubMed] [Google Scholar]
  2. Balmain A., Birnie G. D. Nick translation of mammalian DNA. Biochim Biophys Acta. 1979 Jan 26;561(1):155–166. doi: 10.1016/0005-2787(79)90499-4. [DOI] [PubMed] [Google Scholar]
  3. Birnie G. D., MacPhail E., Young B. D., Getz M. J., Paul J. The diversity of the messenger RNA population in growing Friend cells. Cell Differ. 1974 Nov;3(4):221–232. doi: 10.1016/0045-6039(74)90005-0. [DOI] [PubMed] [Google Scholar]
  4. Chikaraishi D. M., Deeb S. S., Sueoka N. Sequence complexity of nuclear RNAs in adult rat tissues. Cell. 1978 Jan;13(1):111–120. doi: 10.1016/0092-8674(78)90142-3. [DOI] [PubMed] [Google Scholar]
  5. Church R. B., McCarthy B. J. Ribonucleic acid synthesis in regenerating and embryonic liver. I. The synthesis of new species of RNA during regeneration of mouse liver after partial hepatectomy. J Mol Biol. 1967 Feb 14;23(3):459–475. doi: 10.1016/s0022-2836(67)80118-9. [DOI] [PubMed] [Google Scholar]
  6. Church R., McCarthy B. J. Changes in nuclear and cytoplasmic RNA in regenerating mouse liver. Proc Natl Acad Sci U S A. 1967 Oct;58(4):1548–1555. doi: 10.1073/pnas.58.4.1548. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Colbert D. A., Tedeschi M. V., Atryzek V., Fausto N. Diversity of polyadenylated messenger RNA sequences in normal and 12-hr regenerating liver. Dev Biol. 1977 Sep;59(2):111–123. doi: 10.1016/0012-1606(77)90246-9. [DOI] [PubMed] [Google Scholar]
  8. GRISHAM J. W. A morphologic study of deoxyribonucleic acid synthesis and cell proliferation in regenerating rat liver; autoradiography with thymidine-H3. Cancer Res. 1962 Aug;22:842–849. [PubMed] [Google Scholar]
  9. Getz M. J., Birnie G. D., Young B. D., MacPhail E., Paul J. A kinetic estimation of base sequence complexity of nuclear poly(A)-containing RNA in mouse Friend cells. Cell. 1975 Feb;4(2):121–129. doi: 10.1016/0092-8674(75)90118-x. [DOI] [PubMed] [Google Scholar]
  10. Getz M. J., Elder P. K., Benz E. W., Jr, Stephens R. E., Moses H. L. Effect of cell proliferation on levels and diversity of poly(A)-containing mRNA. Cell. 1976 Feb;7(2):255–65. doi: 10.1016/0092-8674(76)90025-8. [DOI] [PubMed] [Google Scholar]
  11. Getz M. J., Reiman H. M., Jr, Siegal G. P., Quinlan T. J., Proper J., Elder P. K., Moses H. L. Gene expression in chemically transformed mouse embryo cells: selective enhancement of the expression of C type RNA tumor virus genes. Cell. 1977 Aug;11(4):909–921. doi: 10.1016/0092-8674(77)90302-6. [DOI] [PubMed] [Google Scholar]
  12. Greene R. F., Fausto N. Analysis of gene expression in regenerating rat liver by hybridization of nuclear and cytoplasmic RNA with DNA. Cancer Res. 1977 Jan;37(1):118–127. [PubMed] [Google Scholar]
  13. Hell A., Birnie G. D., Slimming T. K., Paul J. Controlled fragmentation of DNA by DNase I. Anal Biochem. 1972 Aug;48(2):369–377. doi: 10.1016/0003-2697(72)90089-9. [DOI] [PubMed] [Google Scholar]
  14. Kleiman L., Birnie G. D., Young B. D., Paul J. Comparison of the base-sequence complexities of polysomal and nuclear RNAs in growing Friend erythroleukemia cells. Biochemistry. 1977 Mar 22;16(6):1218–1223. doi: 10.1021/bi00625a029. [DOI] [PubMed] [Google Scholar]
  15. Loeb J. N., Yeung L. L. Free and membrane-bound ribosomes in regenerating rat liver. Biochim Biophys Acta. 1978 Oct 24;520(3):623–629. doi: 10.1016/0005-2787(78)90147-8. [DOI] [PubMed] [Google Scholar]
  16. Mauron A., Spohr G. Frequency distribution of mRNA and pre-mRNA in growing and differentiated Friend cells. Nucleic Acids Res. 1978 Aug;5(8):3013–3032. doi: 10.1093/nar/5.8.3013. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Rolton H. A., Birnie G. D., Paul J. The diversity and specificity of nuclear and polysomal poly(A)+ RNA populations in normal and MSV-transformed cells. Cell Differ. 1977 Jun;6(1):25–39. doi: 10.1016/0045-6039(77)90042-2. [DOI] [PubMed] [Google Scholar]
  18. Savage M. J., Sala-Trepat J. M., Bonner J. Measurement of the complexity and diversity of poly(adenylic acid) containing messenger RNA from rat liver. Biochemistry. 1978 Feb 7;17(3):462–467. doi: 10.1021/bi00596a014. [DOI] [PubMed] [Google Scholar]
  19. Schumm D. E., Webb T. E. Transport of informosomes from isolated nuclei of regenerating rat liver. Biochem Biophys Res Commun. 1972 Sep 5;48(5):1259–1265. doi: 10.1016/0006-291x(72)90847-9. [DOI] [PubMed] [Google Scholar]
  20. Shearer R. W., Smuckler E. A. Altered regulation of the transport of RNA from nucleus to cytoplasm in rat hepatoma cells. Cancer Res. 1972 Feb;32(2):339–342. [PubMed] [Google Scholar]
  21. Strand M., August J. T. Polypeptides of cells transformed by RNA or DNA tumor viruses. Proc Natl Acad Sci U S A. 1977 Jul;74(7):2729–2733. doi: 10.1073/pnas.74.7.2729. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Tedeschi M. V., Colbert D. A., Fausto N. Transcription of the non-repetitive genome in liver hypertrophy and the homology between nuclear RNA of normal and 12 H-regenerating liver. Biochim Biophys Acta. 1978 Dec 21;521(2):641–649. doi: 10.1016/0005-2787(78)90305-2. [DOI] [PubMed] [Google Scholar]
  23. Williams J. G., Hoffman R., Penman S. The extensive homology between mRNA sequences of normal and SV40-transformed human fibroblasts. Cell. 1977 Aug;11(4):901–907. doi: 10.1016/0092-8674(77)90301-4. [DOI] [PubMed] [Google Scholar]
  24. Williams J. G., Penman S. The messenger RNA sequences in growing and resting mouse fibroblasts. Cell. 1975 Oct;6(2):197–206. doi: 10.1016/0092-8674(75)90010-0. [DOI] [PubMed] [Google Scholar]
  25. Young B. D., Birnie G. D. Complexity and specificity of polysomal poly(A+) RNA in mouse tissues. Biochemistry. 1976 Jun 29;15(13):2823–2829. doi: 10.1021/bi00658a019. [DOI] [PubMed] [Google Scholar]

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