Skip to main content
NCBI home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1979;6(6):2209–2216. doi: 10.1093/nar/6.6.2209

Incorporation of lysine into Y base of phenylalanine tRNA in Vero cells.

R G Pergolizzi, D L Engelhardt, D Grunberger
PMCID: PMC327846  PMID: 111226

Abstract

Vero cells, a line derived from African green monkey kidney, contains a hypermodified base, called Y, adjacent to the 3' end of the anticodon of tRNAPhe. Two types of evidence are presented suggesting that lysine is involved in biosynthesis of Y base in these cells. First, when Vero cells are starved for lysine, a new, early-eluting species of tRNAPhe which lacks the fully modified Y base can be detected by reversed phase chromatography (RPC-5). After addition of lysine to the medium, this new species disappears. Second, when these cells are grown in low-lysine medium and then exposed to [3H]lysine, radioactivity from the lysine comigrates with tRNAPhe. The Y base can be selectively excised from tRNAPhe by incubation at pH 2.9, and extracted into ethyl acetate. Thin-layer chromatography of acid-excised material from these cells reveals that lysine-derived radioactivity comigrates with genuine Y base from calf liver tRNAPhe and the acid-excised tRNA no longer contains radioactivity. These results are consistent with the model that lysine is a structural precursor of Y base in tRNAPhe of Vero cells.

Full text

PDF
2209

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Blobstein S. H., Grunberger D., Weinstein I. B., Nakanishi K. Isolation and structure determination of the fluorescent base from bovine liver phenylalanine transfer ribonucleic acid. Biochemistry. 1973 Jan 16;12(2):188–193. doi: 10.1021/bi00726a002. [DOI] [PubMed] [Google Scholar]
  2. Goldschmidt B. M., Blazej T. P., Van Duuren B. L. The reaction of guanosine and deoxyguanosine with glycidaldehyde. Tetrahedron Lett. 1968 Feb;13:1583–1586. doi: 10.1016/s0040-4039(01)99007-0. [DOI] [PubMed] [Google Scholar]
  3. Grunberger D., Weinstein I. B., Mushinski J. F. Deficiency of the Y base in a hepatoma phenylalanine tRNA. Nature. 1975 Jan 3;253(5486):66–67. doi: 10.1038/253066a0. [DOI] [PubMed] [Google Scholar]
  4. Kuchino Y., Borek E. Changes in transfer RNA's in human malignant trophoblastic cells (BeWo line). Cancer Res. 1976 Aug;36(8):2932–2936. [PubMed] [Google Scholar]
  5. Kuchino Y., Borek E. Tumour-specific phenylalanine tRNA contains two supernumerary methylated bases. Nature. 1978 Jan 12;271(5641):126–129. doi: 10.1038/271126a0. [DOI] [PubMed] [Google Scholar]
  6. Lee G. T., Engelhardt D. L. Protein metabolism during growth of Vero Cells. J Cell Physiol. 1977 Aug;92(2):293–301. doi: 10.1002/jcp.1040920218. [DOI] [PubMed] [Google Scholar]
  7. Li H. J., Nakanishi K., Grunberger D., Weinstein I. B. Biosynthetic studies of the Y base in yeast phenylalanine tRNA. Incorporation of guanine. Biochem Biophys Res Commun. 1973 Dec 10;55(3):818–823. doi: 10.1016/0006-291x(73)91217-5. [DOI] [PubMed] [Google Scholar]
  8. Münch H. J., Thiebe R. Biosynthesis of the nucleoside Y in yeast tRNAPhe: incorporation of the 3-amino-3-carboxypropyl-group from methionine. FEBS Lett. 1975 Mar 1;51(1):257–258. doi: 10.1016/0014-5793(75)80900-8. [DOI] [PubMed] [Google Scholar]
  9. Pearson R. L., Weiss J. F., Kelmers A. D. Improved separation of transfer RNA's on polychlorotrifuoroethylene-supported reversed-phase chromatography columns. Biochim Biophys Acta. 1971 Feb 11;228(3):770–774. doi: 10.1016/0005-2787(71)90748-9. [DOI] [PubMed] [Google Scholar]
  10. Pergolizzi R. G., Engelhardt D. L., Grunberger D. Formation of phenylalanine transfer RNA lacking the wye base in Vero cells during methionine starvation. J Biol Chem. 1978 Sep 25;253(18):6341–6343. [PubMed] [Google Scholar]
  11. ROTHSTEIN M., GREENBERG D. M. The metabolism of DL-pipecolic acid-2-C14. J Biol Chem. 1960 Mar;235:714–718. [PubMed] [Google Scholar]
  12. Thiebe R., Zachau H. G. A specific modification next to the anticodon of phenylalanine transfer ribonucleic acid. Eur J Biochem. 1968 Sep 24;5(4):546–555. doi: 10.1111/j.1432-1033.1968.tb00404.x. [DOI] [PubMed] [Google Scholar]
  13. Todaro G. J., Aaronson S. A., Rands E. Rapid detection of mycoplasma-infected cell cultures. Exp Cell Res. 1971 Mar;65(1):256–257. doi: 10.1016/s0014-4827(71)80077-0. [DOI] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES