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. 1979 Jun 11;6(7):2435–2452. doi: 10.1093/nar/6.7.2435

Cloning of an almost full-length chicken conalbumin double-stranded cDNA.

M Cochet, F Perrin, F Gannon, A Krust, P Chambon, G S McKnight, D C Lee, K E Mayo, R Palmiter
PMCID: PMC327864  PMID: 88720

Abstract

Chicken conalbumin double-stranded cDNA (con-dscDNA) was synthesized from a laying hen oviduct mRNA preparation enriched for conalbumin mRNA (con-mRNA). The dscDNA was inserted by blunt-end ligation into the Sal I site of plasmid pBR322 which had been repaired with DNA polymerase I to create Taq I sites on each side of the inserted fragment. After bacterial transformation, one hybrid recombinant, pBR322-con1, which contains the largest inserted dscDNA (about 2350 bp) was shown to hybridize specifically to the RNA which is translated into conalbumin. Electron microscopic examination of hybrid molecules between con-mRNA and pBR322-con1 DNA indicate that the inserted con-dscDNA is an almost full-length double-stranded transcript of conalbumin mRNA.

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Selected References

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