Skip to main content
NCBI home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1979 Jun 11;6(7):2583–2599. doi: 10.1093/nar/6.7.2583

Studies on gene control regions X. The effect of specific adenine-thymine transversions on the lac repressor-lac operator interaction.

H S Sista, R T Loder, M H Caruthers
PMCID: PMC327873  PMID: 379824

Abstract

Chemical and enzymatic methods were used to synthesize a transition (AT to GC) and a transversion (AT to TA) at a lac operator site known to interact with lac repressor through the thymine 5 methyl group. These operators also contained a poly(dA) . poly(dT) tail 8 to 12 base pairs in length at one end. Results suggest that the steric constraints of lac repressor relative to the position of the 5 methyl group are quite critical. For example a seven fold reduction in stability was observed for the transversion. Results also suggest that the operator spans at least 21 base pairs.

Full text

PDF
2583

Images in this article

2588Image
on p.2588
2590Image
on p.2590

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arnott S., Selsing E. Structures for the polynucleotide complexes poly(dA) with poly (dT) and poly(dT) with poly(dA) with poly (dT). J Mol Biol. 1974 Sep 15;88(2):509–521. doi: 10.1016/0022-2836(74)90498-7. [DOI] [PubMed] [Google Scholar]
  2. Bahl C. P., Wu R., Stawinsky J., Narang S. A. Minimal length of the lactose operator sequence for the specific recognition by the lactose repressor. Proc Natl Acad Sci U S A. 1977 Mar;74(3):966–970. doi: 10.1073/pnas.74.3.966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Burd J. F., Wartell R. M., Dodgson J. B., Wells R. D. Transmission of stability (telestability) in deoxyribonucleic acid. Physical and enzymatic studies on the duplex block polymer d(C15A15) - d(T15G15). J Biol Chem. 1975 Jul 10;250(13):5109–5113. [PubMed] [Google Scholar]
  4. Goeddel D. V., Yansura D. G., Caruthers M. H. Binding of synthetic lactose operator DNAs to lactose represessors. Proc Natl Acad Sci U S A. 1977 Aug;74(8):3292–3296. doi: 10.1073/pnas.74.8.3292. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Goeddel D. V., Yansura D. G., Caruthers M. H. How lac repressor recognizes lac operator. Proc Natl Acad Sci U S A. 1978 Aug;75(8):3578–3582. doi: 10.1073/pnas.75.8.3578. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Goeddel D. V., Yansura D. G., Caruthers M. H. Studies on gene control regions. 1. Chemical synthesis of lactose operator deoxyribonucleic acid segments. Biochemistry. 1977 May 3;16(9):1765–1772. doi: 10.1021/bi00628a001. [DOI] [PubMed] [Google Scholar]
  7. Goeddel D. V., Yansura D. G., Caruthers M. H. Studies on gene control regions. VI. The 5- methyl of thymine, a lac repressor recognition site. Nucleic Acids Res. 1977 Sep;4(9):3039–3054. doi: 10.1093/nar/4.9.3039. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Goeddel D. V., Yansura D. G., Winston C., Caruthers M. H. Studies on gene control regions. VII. Effect of 5-bromuracil-substituted lac operators on the lac operator-lac repressor interaction. J Mol Biol. 1978 Aug 25;123(4):661–687. doi: 10.1016/0022-2836(78)90211-5. [DOI] [PubMed] [Google Scholar]
  9. Lillehaug J. R., Kleppe R. K., Kleppe K. Phosphorylation of double-stranded DNAs by T4 polynucleotide kinase. Biochemistry. 1976 May 4;15(9):1858–1865. doi: 10.1021/bi00654a011. [DOI] [PubMed] [Google Scholar]
  10. Lin S. Y., Riggs A. D. Lac repressor binding to non-operator DNA: detailed studies and a comparison of eequilibrium and rate competition methods. J Mol Biol. 1972 Dec 30;72(3):671–690. doi: 10.1016/0022-2836(72)90184-2. [DOI] [PubMed] [Google Scholar]
  11. Ogata R., Gilbert W. Contacts between the lac repressor and the thymines in the lac operator. Proc Natl Acad Sci U S A. 1977 Nov;74(11):4973–4976. doi: 10.1073/pnas.74.11.4973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Riggs A. D., Bourgeois S., Cohn M. The lac repressor-operator interaction. 3. Kinetic studies. J Mol Biol. 1970 Nov 14;53(3):401–417. doi: 10.1016/0022-2836(70)90074-4. [DOI] [PubMed] [Google Scholar]
  13. Riggs A. D., Lin S., Wells R. D. Lac repressor binding to synthetic DNAs of defined nucleotide sequence. Proc Natl Acad Sci U S A. 1972 Mar;69(3):761–764. doi: 10.1073/pnas.69.3.761. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Riggs A. D., Suzuki H., Bourgeois S. Lac repressor-operator interaction. I. Equilibrium studies. J Mol Biol. 1970 Feb 28;48(1):67–83. doi: 10.1016/0022-2836(70)90219-6. [DOI] [PubMed] [Google Scholar]
  15. Roychoudhury R., Jay E., Wu R. Terminal labeling and addition of homopolymer tracts to duplex DNA fragments by terminal deoxynucleotidyl transferase. Nucleic Acids Res. 1976 Jan;3(1):101–116. doi: 10.1093/nar/3.1.101. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Sanger F., Donelson J. E., Coulson A. R., Kössel H., Fischer D. Use of DNA polymerase I primed by a synthetic oligonucleotide to determine a nucleotide sequence in phage fl DNA. Proc Natl Acad Sci U S A. 1973 Apr;70(4):1209–1213. doi: 10.1073/pnas.70.4.1209. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Sgaramella V., Khorana H. G. CXII. Total synthesis of the structural gene for an alanine transfer RNA from yeast. Enzymic joining of the chemically synthesized polydeoxynucleotides to form the DNA duplex representing nucleotide sequence 1 to 20. J Mol Biol. 1972 Dec 28;72(2):427–444. doi: 10.1016/0022-2836(72)90155-6. [DOI] [PubMed] [Google Scholar]
  18. Yansura D. G., Goeddel D. V., Caruthers M. H. Studies on gene control regions. 2. Enzymatic joining of chemically synthesized lactose operator deoxyribonucleic acid segments. Biochemistry. 1977 May 3;16(9):1772–1780. doi: 10.1021/bi00628a002. [DOI] [PubMed] [Google Scholar]
  19. Yansura D. G., Goeddel D. V., Cribbs D. L., Caruthers M. H. Studies of gene control regions. III. Binding of synthetic and modified synthetic lac operator DNAs to lactose repressor. Nucleic Acids Res. 1977 Mar;4(3):723–737. doi: 10.1093/nar/4.3.723. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES