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. 1979 Jun 25;6(8):2707–2715. doi: 10.1093/nar/6.8.2707

Cloning of double stranded DNAs derived from polysomal mRNA of maize endosperm: isolation and characterisation of zein clones.

U Weinand, C Brüschke, G Feix
PMCID: PMC327887  PMID: 461201

Abstract

Starting from polysomal RNA of developing maize endosperm and applying the cloning procedure of integrating dsDNA into the Pst I site of plasmid pBR 322, clones containing sequences complementary to endosperm mRNAs were obtained. 25 per cent of these clones were identified as containing zein specific DNA sequences which hybridized either with the zein mRNA coding for the 22 000 Mr protein or with the zein mRNA coding for the 19 000 Mr protein. The zein-specific DNA inserts of the recombinant plasmids were further characterized by restriction enzyme analysis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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