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. 1979 Jul 11;6(9):3119–3131. doi: 10.1093/nar/6.9.3119

Biological activity of the intact and cleaved DNA of the simian adenovirus 7.

T I Ponomareva, N A Grodnitskaya, E E Goldberg, N M Chaplygina, B S Naroditsky, T I Tikchonenko
PMCID: PMC327920  PMID: 114980

Abstract

Only the deproteinized DNA preparations of the simian adenovirus of the type 7 (SA 7) exhibited transforming and tumorigenic activity. The complex of the SA7 DNA with terminal protein (TP) did not exhibit either transforming or tumorigenic activity in cell cultures. In contrast to the transforming potential the infectious titers of the DNA - TP complex for the monkey kidney cells were 30-50 times higher than those of pure DNA. Cleavage of the SA7 DNA by specific endonucleases enhanced the tumorigenic potential of pure DNA, suppressed its infectivity and did not affect the lack of transformation capacity of the DNA - TP complex. The onc-gene was localized in the left terminal fragment with the minimal size 4,3x10(6)D in the case of R.Sal I. The tumorigenic activity was found to decrease with an increase in the size of the DNA fragment containing the onc-gene.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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