Abstract
We have determined the nucleotide sequence of a segment of Saccharomyces cerevisiae mtDNA that contains the structural genes for a cysteine-tRNA and a histidine-tRNA. The genes are approximately 85 bp apart, they do not contain intervening sequences or sequences coding for the 3'-CCA terminus and they are surrounded by nearly pure AT segments. The tRNAs deduced are very AT-rich, 74 and 75 nucleotides long, respectively, and contain one or more unusual features not found in tRNAs from other sources.
Full text
PDF











Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Altman S. Biosynthesis of transfer RNA in Escherichia coli. Cell. 1975 Jan;4(1):21–29. doi: 10.1016/0092-8674(75)90129-4. [DOI] [PubMed] [Google Scholar]
- Borst P., Grivell L. A. The mitochondrial genome of yeast. Cell. 1978 Nov;15(3):705–723. doi: 10.1016/0092-8674(78)90257-x. [DOI] [PubMed] [Google Scholar]
- Borst P. Mitochondrial nucleic acids. Annu Rev Biochem. 1972;41:333–376. doi: 10.1146/annurev.bi.41.070172.002001. [DOI] [PubMed] [Google Scholar]
- Bos J. L., Heyting C., Borst P., Arnberg A. C., Van Bruggen E. F. An insert in the single gene for the large ribosomal RNA in yeast mitochondrial DNA. Nature. 1978 Sep 28;275(5678):336–338. doi: 10.1038/275336a0. [DOI] [PubMed] [Google Scholar]
- Deutsch J., Dujon B., Netter P., Petrochilo E., Slonimski P. P., Bolotin-Fukuhara M., Coen D. Mitochondrial genetics. VI. The petite mutation in Saccharomyces cerevisiae: interrelations between the loss of the p+ factor and the loss of the drug resistance mitochondrial genetic markers. Genetics. 1974 Feb;76(2):195–219. doi: 10.1093/genetics/76.2.195. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Donis-Keller H., Maxam A. M., Gilbert W. Mapping adenines, guanines, and pyrimidines in RNA. Nucleic Acids Res. 1977 Aug;4(8):2527–2538. doi: 10.1093/nar/4.8.2527. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gauss D. H., Grüter F., Sprinzl M. Compilation of tRNA sequences. Nucleic Acids Res. 1979 Jan;6(1):r1–r19. [PMC free article] [PubMed] [Google Scholar]
- Goodman H. M., Olson M. V., Hall B. D. Nucleotide sequence of a mutant eukaryotic gene: the yeast tyrosine-inserting ochre suppressor SUP4-o. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5453–5457. doi: 10.1073/pnas.74.12.5453. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Heckman J. E., Alzner-Deweerd B., RajBhandary U. L. Interesting and unusual features in the sequence of Neurospora crassa mitochondrial tyrosine transfer RNA. Proc Natl Acad Sci U S A. 1979 Feb;76(2):717–721. doi: 10.1073/pnas.76.2.717. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Heckman J. E., Hecker L. I., Schwartzbach S. D., Barnett W. E., Baumstark B., RajBhandary U. L. Structure and function of initiator methionine tRNA from the mitochondria of Neurospora crassa. Cell. 1978 Jan;13(1):83–95. doi: 10.1016/0092-8674(78)90140-x. [DOI] [PubMed] [Google Scholar]
- Hensgens L. A., Grivell L. A., Borst P., Bos J. L. Nucleotide sequence of the mitochondrial structural gene for subunit 9 of yeast ATPase complex. Proc Natl Acad Sci U S A. 1979 Apr;76(4):1663–1667. doi: 10.1073/pnas.76.4.1663. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Heyting C., Meijlink F. C., Verbeet M. P., Sanders J. P., Bos J. L., Borst P. Fine structure of the 21S ribosomal RNA region on yeast mitochondria DNA. I. Construction of the physical map and localization of the cistron for the 21S mitochondrial ribosomal RNA. Mol Gen Genet. 1979 Jan 11;168(3):231–246. doi: 10.1007/BF00271496. [DOI] [PubMed] [Google Scholar]
- Heyting C., Talen J. L., Weijers P. J., Borst P. Fine structure of the 21S ribosomal RNA region on yeast mitochondrial DNA. II. The organization of sequences in petite mitochondrial DNAs carrying genetic markers from the 21S region. Mol Gen Genet. 1979 Jan 11;168(3):251–277. doi: 10.1007/BF00271497. [DOI] [PubMed] [Google Scholar]
- Kim S. H., Suddath F. L., Quigley G. J., McPherson A., Sussman J. L., Wang A. H., Seeman N. C., Rich A. Three-dimensional tertiary structure of yeast phenylalanine transfer RNA. Science. 1974 Aug 2;185(4149):435–440. doi: 10.1126/science.185.4149.435. [DOI] [PubMed] [Google Scholar]
- Maat J., Smith A. J. A method for sequencing restriction fragments with dideoxynucleoside triphosphates. Nucleic Acids Res. 1978 Dec;5(12):4537–4545. doi: 10.1093/nar/5.12.4537. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Martin N. C., Rabinowitz M., Fukuhara H. Yeast mitochondrial DNA specifies tRNA for 19 amino acids. Deletion mapping of the tRNA genes. Biochemistry. 1977 Oct 18;16(21):4672–4677. doi: 10.1021/bi00640a022. [DOI] [PubMed] [Google Scholar]
- Martin N. C., rabinowitz M. Mitochondrial transfer RNAs in yeast: identification of isoaccepting transfer RNAs. Biochemistry. 1978 May 2;17(9):1628–1634. doi: 10.1021/bi00602a008. [DOI] [PubMed] [Google Scholar]
- Martin R. P., Sibler A. P., Schneller J. M., Keith G., Stahl A. J., Dirheimer G. Primary structure of yeast mitochondrial DNA-coded phenylalanine-tRNA. Nucleic Acids Res. 1978 Dec;5(12):4579–4592. doi: 10.1093/nar/5.12.4579. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Martin R., Schneller J. M., Stahl A. J., Dirheimer G. Studies of odd bases in yeast mitochondrial tRNA: II. Characterization of rare nucleosides. Biochem Biophys Res Commun. 1976 Jun 7;70(3):997–1002. doi: 10.1016/0006-291x(76)90690-2. [DOI] [PubMed] [Google Scholar]
- Maxam A. M., Gilbert W. A new method for sequencing DNA. Proc Natl Acad Sci U S A. 1977 Feb;74(2):560–564. doi: 10.1073/pnas.74.2.560. [DOI] [PMC free article] [PubMed] [Google Scholar]
- McClain W. H., Seidman J. G., Schmidt F. J. Evolution of the biosynthesis of 3'-terminal C-C-A residues in T-even bacteriophage transfer RNAs. J Mol Biol. 1978 Mar 15;119(4):519–536. doi: 10.1016/0022-2836(78)90200-0. [DOI] [PubMed] [Google Scholar]
- Moorman A. F., Grivell L. A., Lamie F., Smits H. L. Identification of mitochondrial gene products by DNA-directed protein synthesis in vitro. Biochim Biophys Acta. 1978 Apr 27;518(2):351–365. doi: 10.1016/0005-2787(78)90192-2. [DOI] [PubMed] [Google Scholar]
- Penefsky H. S. Reversible binding of Pi by beef heart mitochondrial adenosine triphosphatase. J Biol Chem. 1977 May 10;252(9):2891–2899. [PubMed] [Google Scholar]
- Robertus J. D., Ladner J. E., Finch J. T., Rhodes D., Brown R. S., Clark B. F., Klug A. Structure of yeast phenylalanine tRNA at 3 A resolution. Nature. 1974 Aug 16;250(467):546–551. doi: 10.1038/250546a0. [DOI] [PubMed] [Google Scholar]
- Seeburg P. H., Shine J., Martial J. A., Ullrich A., Baxter J. D., Goodman H. M. Nucleotide sequence of part of the gene for human chorionic somatomammotropin: purification of DNA complementary to predominant mRNA species. Cell. 1977 Sep;12(1):157–165. doi: 10.1016/0092-8674(77)90193-3. [DOI] [PubMed] [Google Scholar]
- Singer C. E., Smith G. R. Histidine regulation in Salmonella typhimurium. 13. Nucleotide sequence of histidine transfer ribonucleic acid. J Biol Chem. 1972 May 25;247(10):2989–3000. [PubMed] [Google Scholar]
- Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
- Valenzuela P., Venegas A., Weinberg F., Bishop R., Rutter W. J. Structure of yeast phenylalanine-tRNA genes: an intervening DNA segment within the region coding for the tRNA. Proc Natl Acad Sci U S A. 1978 Jan;75(1):190–194. doi: 10.1073/pnas.75.1.190. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Van Ommen G. J., Groot G. S., Borst P. Fine structure physical mapping of 4S RNA genes on mitochondrial DNA of Saccharomyces cerevisiae. Mol Gen Genet. 1977 Sep 9;154(3):255–262. doi: 10.1007/BF00571280. [DOI] [PubMed] [Google Scholar]