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. 1979 Aug 10;6(11):3651–3660. doi: 10.1093/nar/6.11.3651

Isolation of a single polypeptide leucyl-tRNA synthetase from bakers' yeast.

C S Lin, R Irwin, J G Chirikjian
PMCID: PMC327963  PMID: 386276

Abstract

A single polypeptide of leucyl-tRNA synthetase (LRS) has been purified from budding bakers' yeast by a modification of the procedure published earlier. On denaturing polyacrylamide gel electrophoresis LRS was one band corresponding to molecular weight of 120,000 +/- 5,000 daltons. Variable amounts of LRS with a similar molecular weight but which dissociated into equal subunits of 58,000 were also isolated. The affinities (KM) for substrates for this form of the enzyme were similar to those previously reported for the dimeric form of the enzyme.

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Selected References

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  1. Bruton C. J., Hartley B. S. The subunits of methionyl-transfer-ribonucleic acid synthetase. Biochem J. 1970 Apr;117(2):18P–19P. doi: 10.1042/bj1170018p. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Calendar R., Berg P. Purification and physical characterization of tyrosyl ribonucleic acid synthetases from Escherichia coli and Bacillus subtilis. Biochemistry. 1966 May;5(5):1681–1690. doi: 10.1021/bi00869a033. [DOI] [PubMed] [Google Scholar]
  3. Chirikjian J. G., Kanagalingam K., Lau E., Fresco J. R. Purification and properties of leucyl-tRNA synthetase from Bakers' yeast. J Biol Chem. 1973 Feb 10;248(3):1074–1079. [PubMed] [Google Scholar]
  4. DAVIS B. J. DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS. Ann N Y Acad Sci. 1964 Dec 28;121:404–427. doi: 10.1111/j.1749-6632.1964.tb14213.x. [DOI] [PubMed] [Google Scholar]
  5. Godson G. N. A simple apparatus for rapid isoelectrofocusing of multiple samples on a micro scale. Anal Biochem. 1970 May;35(1):66–76. doi: 10.1016/0003-2697(70)90010-2. [DOI] [PubMed] [Google Scholar]
  6. Gros C., Lemaire G., Van Rapenbusch R., Labouesse B. The subunit structure of tryptophanyl transfer ribonucleic acid synthetase from beef pancreas. J Biol Chem. 1972 May 10;247(9):2931–2943. [PubMed] [Google Scholar]
  7. Kern D., Giegé R., Robre-Saul S., Boulanger Y., Ebel J. P. Complete purification and studies on the structural and kinetic properties of two forms of yeast valyl-tRNA synthetase. Biochimie. 1975;57(10):1167–1176. doi: 10.1016/s0300-9084(76)80579-2. [DOI] [PubMed] [Google Scholar]
  8. Kućan Z., Chambers R. W. Purification of tyrosine: tRNA ligase from Saccharomyces cerevisiae alphaS288C. J Biochem. 1973 Apr;73(4):811–819. doi: 10.1093/oxfordjournals.jbchem.a130144. [DOI] [PubMed] [Google Scholar]
  9. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  10. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  11. Lemaire G., Gros C., Epely S., Kaminski M., Labouesse B. Multiple forms of tryptophanyl-tRNA synthetase from beef pancreas. Eur J Biochem. 1975 Feb 3;51(1):237–252. doi: 10.1111/j.1432-1033.1975.tb03924.x. [DOI] [PubMed] [Google Scholar]
  12. Lin C. S., Irwin R., Chirikjian J. G. Kinetic studies of leucyl transfer RNA synthetase from bakers' yeast. Order of addition of substrates and release of products. J Biol Chem. 1975 Dec 25;250(24):9299–9303. [PubMed] [Google Scholar]
  13. MARTIN R. G., AMES B. N. A method for determining the sedimentation behavior of enzymes: application to protein mixtures. J Biol Chem. 1961 May;236:1372–1379. [PubMed] [Google Scholar]
  14. Murasugi A., Hayashi H. Purification and properties of leucyl-tRNA synthetase from Candida utilis. Eur J Biochem. 1975 Sep 1;57(1):169–175. doi: 10.1111/j.1432-1033.1975.tb02287.x. [DOI] [PubMed] [Google Scholar]
  15. Paradies H. H. Two enzymatically active forms of valyl-tRNA-synthetase from E. coli. Biochem Biophys Res Commun. 1975 Jun 16;64(4):1253–1262. doi: 10.1016/0006-291x(75)90827-x. [DOI] [PubMed] [Google Scholar]
  16. Schmidt J., Wang R., Stanfield S., Reid B. R. Yeast phenylalanyl transfer ribonucleic acid synthetase. Purification, molecular weight, and subunit structure. Biochemistry. 1971 Aug 17;10(17):3264–3268. doi: 10.1021/bi00793a016. [DOI] [PubMed] [Google Scholar]
  17. Sternbach H., von der Haar F., Schlimme E., Gaertner E., Cramer F. Isolation and properties of tRNA nucleotidyl transferase from yeast. Eur J Biochem. 1971 Sep 24;22(2):166–172. doi: 10.1111/j.1432-1033.1971.tb01528.x. [DOI] [PubMed] [Google Scholar]
  18. Surguchov A. P., Surguchova I. G. Two enzymically active forms of glycyl-tRNA synthetase from Bacillus brevis. Purification and properties. Eur J Biochem. 1975 May;54(1):175–184. doi: 10.1111/j.1432-1033.1975.tb04127.x. [DOI] [PubMed] [Google Scholar]

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