Skip to main content
PMC home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1979 Aug 24;6(12):3805–3819. doi: 10.1093/nar/6.12.3805

Molecular cloning of three major sequence species from Rainbow trout protamine mRNA.

J R Jenkins, J O Bishop, P H Butterworth
PMCID: PMC327979  PMID: 493125

Abstract

Double stranded cDNA molecules complementary to purified Rainbow trout protamine mRNA have been cloned in the bacterial plasmid pBR322. In order to circumvent the problems associated with a heterogeneous cDNA probe when identifying recombinants, a comparative hybridisation technique was used which can resolve between closely related cloned sequences. Using this technique, selected recombinants were shown to carry sequences corresponding to separate major fractions of protamine mRNA. Partial nucleotide sequences of the inserts in two clones confirms this conclusion.

Full text

PDF
3805

Images in this article

3814Image
on p.3814

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ando T., Watanabe S. A new method for fractionation of protamines and the amino acid sequences of salmine and three components of iridine. Int J Protein Res. 1969;1(3):221–224. doi: 10.1111/j.1399-3011.1969.tb01646.x. [DOI] [PubMed] [Google Scholar]
  2. Bishop J. O., Beckmann J. S., Campo M. S., Hastie N. D., Izquierdo M., Perlman S. DNA-RNA hybridization. Philos Trans R Soc Lond B Biol Sci. 1975 Nov 6;272(915):147–157. doi: 10.1098/rstb.1975.0077. [DOI] [PubMed] [Google Scholar]
  3. Bishop J. O., Freeman K. B. DNA sequences neighboring the duck hemoglobin genes. Cold Spring Harb Symp Quant Biol. 1974;38:707–716. doi: 10.1101/sqb.1974.038.01.076. [DOI] [PubMed] [Google Scholar]
  4. Clewell D. B., Helinski D. R. Properties of a supercoiled deoxyribonucleic acid-protein relaxation complex and strand specificity of the relaxation event. Biochemistry. 1970 Oct 27;9(22):4428–4440. doi: 10.1021/bi00824a026. [DOI] [PubMed] [Google Scholar]
  5. Gedamu L., Dixon G. H. Purification and properties of biologically active rainbow trout testis protamine mRNA. J Biol Chem. 1976 Mar 10;251(5):1455–1463. [PubMed] [Google Scholar]
  6. Jenkins J. R. Sequence divergence of rainbow trout protamine mRNAs; comparison of coding and non-coding nucleotide sequences in three protamine cDNA plasmids. Nature. 1979 Jun 28;279(5716):809–811. doi: 10.1038/279809a0. [DOI] [PubMed] [Google Scholar]
  7. Levy W. B., Dixon G. H. Reiteration frequency of the protamine genes in rainbow trout (Salmo gairdnerii). J Biol Chem. 1977 Nov 25;252(22):8062–8065. [PubMed] [Google Scholar]
  8. Maniatis T., Kee S. G., Efstratiadis A., Kafatos F. C. Amplification and characterization of a beta-globin gene synthesized in vitro. Cell. 1976 Jun;8(2):163–182. doi: 10.1016/0092-8674(76)90001-5. [DOI] [PubMed] [Google Scholar]
  9. Roberts B. E., Paterson B. M. Efficient translation of tobacco mosaic virus RNA and rabbit globin 9S RNA in a cell-free system from commercial wheat germ. Proc Natl Acad Sci U S A. 1973 Aug;70(8):2330–2334. doi: 10.1073/pnas.70.8.2330. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Sakai M., Fujii-Kuriyama Y., Muramatsu M. Number and frequency of protamine genes in rainbow trout testis. Biochemistry. 1978 Dec 12;17(25):5510–5515. doi: 10.1021/bi00618a028. [DOI] [PubMed] [Google Scholar]
  11. Sutcliffe J. G. Nucleotide sequence of the ampicillin resistance gene of Escherichia coli plasmid pBR322. Proc Natl Acad Sci U S A. 1978 Aug;75(8):3737–3741. doi: 10.1073/pnas.75.8.3737. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES