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. 1979 Aug 24;6(12):3923–3931. doi: 10.1093/nar/6.12.3923

The nucleotide sequence of the 5' terminus of vesicular stomatitis virus RNA.

B L Semler, J Perrault, J J Holland
PMCID: PMC327987  PMID: 226942

Abstract

We have determined the nucleotide sequence for the first 50 nucleotides at the 5' terminus of vesicular stomatitis virus (VSV) genome RNA. This sequence is identical to that of the in vitro RNA polymerase product synthesized by defective interfering (DI) particles of VSV. These results confirm previous conclusions rengarding DI and standard viral terminal sequences based on hybridization studies and earlier sequencing of the DI polymerase product RNA.

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Selected References

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  1. Berns K. I., Kelly T. J., Jr Letter: Visualization of the inverted terminal repetition in adeno-associated virus DNA. J Mol Biol. 1974 Jan 15;82(2):267–271. doi: 10.1016/0022-2836(74)90344-1. [DOI] [PubMed] [Google Scholar]
  2. Colonno R. J., Banerjee A. K. A unique RNA species involved in initiation of vesicular stomatitis virus RNA transcription in vitro. Cell. 1976 Jun;8(2):197–204. doi: 10.1016/0092-8674(76)90003-9. [DOI] [PubMed] [Google Scholar]
  3. Colonno R. J., Banerjee A. K. Complete nucleotide sequence of the leader RNA synthesized in vitro by vesicular stomatitis virus. Cell. 1978 Sep;15(1):93–101. doi: 10.1016/0092-8674(78)90085-5. [DOI] [PubMed] [Google Scholar]
  4. Donis-Keller H., Maxam A. M., Gilbert W. Mapping adenines, guanines, and pyrimidines in RNA. Nucleic Acids Res. 1977 Aug;4(8):2527–2538. doi: 10.1093/nar/4.8.2527. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Doyle M., Holland J. J. Prophylaxis and immunization in mice by use of virus-free defective T particles to protect against intracerebral infection by vesicular stomatitis virus. Proc Natl Acad Sci U S A. 1973 Jul;70(7):2105–2108. doi: 10.1073/pnas.70.7.2105. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Garon C. F., Berry K. W., Rose J. A. A unique form of terminal redundancy in adenovirus DNA molecules. Proc Natl Acad Sci U S A. 1972 Sep;69(9):2391–2395. doi: 10.1073/pnas.69.9.2391. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Grafstrom R. H., Alwine J. C., Steinhart W. L., Hill C. W., Hyman R. W. The terminal repetition of herpes simplex virus DNA. Virology. 1975 Sep;67(1):144–157. doi: 10.1016/0042-6822(75)90412-2. [DOI] [PubMed] [Google Scholar]
  8. Hefti E., Bishop D. H. The 5' nucleotide sequence of vesicular stomatitis viral RNA. J Virol. 1975 Jan;15(1):90–96. doi: 10.1128/jvi.15.1.90-96.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hsu M. T., Kung H. J., Davidson N. An electron microscope study of Sindbis virus RNA. Cold Spring Harb Symp Quant Biol. 1974;38:943–950. doi: 10.1101/sqb.1974.038.01.096. [DOI] [PubMed] [Google Scholar]
  10. Huang A. S., Manders E. K. Ribonucleic acid synthesis of vesicular stomatitis virus. IV. Transcription by standard virus in the presence of defective interfering particles. J Virol. 1972 Jun;9(6):909–916. doi: 10.1128/jvi.9.6.909-916.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Huang A. S. Viral pathogenesis and molecular biology. Bacteriol Rev. 1977 Dec;41(4):811–821. doi: 10.1128/br.41.4.811-821.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Keene J. D., Schubert M., Lazzarini R. A., Rosenberg M. Nucleotide sequence homology at the 3' termini of RNA from vesicular stomatitis virus and its defective interfering particles. Proc Natl Acad Sci U S A. 1978 Jul;75(7):3225–3229. doi: 10.1073/pnas.75.7.3225. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Kennedy S. I. Sequence relationships between the genome and the intracellular RNA species of standard and defective-interfering Semliki Forest virus. J Mol Biol. 1976 Dec;108(2):491–511. doi: 10.1016/s0022-2836(76)80132-5. [DOI] [PubMed] [Google Scholar]
  14. Koczot F. J., Carter B. J., Garon C. F., Rose J. A. Self-complementarity of terminal sequences within plus or minus strands of adenovirus-associated virus DNA. Proc Natl Acad Sci U S A. 1973 Jan;70(1):215–219. doi: 10.1073/pnas.70.1.215. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Kolakofsky D. Isolation and characterization of Sendai virus DI-RNAs. Cell. 1976 Aug;8(4):547–555. doi: 10.1016/0092-8674(76)90223-3. [DOI] [PubMed] [Google Scholar]
  16. Leppert M., Kort L., Kolakofsky D. Further characterization of Sendai virus DI-RNAs: a model for their generation. Cell. 1977 Oct;12(2):539–552. doi: 10.1016/0092-8674(77)90130-1. [DOI] [PubMed] [Google Scholar]
  17. Maxam A. M., Gilbert W. A new method for sequencing DNA. Proc Natl Acad Sci U S A. 1977 Feb;74(2):560–564. doi: 10.1073/pnas.74.2.560. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. McMaster G. K., Carmichael G. G. Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange. Proc Natl Acad Sci U S A. 1977 Nov;74(11):4835–4838. doi: 10.1073/pnas.74.11.4835. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Mudd J. A., Summers D. F. Polysomal ribonucleic acid of vesicular stomatitis virus-infected HeLa cells. Virology. 1970 Dec;42(4):958–968. doi: 10.1016/0042-6822(70)90344-2. [DOI] [PubMed] [Google Scholar]
  20. Perrault J., Holland J. J. Absence of transcriptase activity or transcription-inhibiting ability in defective interfering particles of vesicular stomatitis virus. Virology. 1972 Oct;50(1):150–170. [PubMed] [Google Scholar]
  21. Perrault J., Leavitt R. W. Inverted complementary terminal sequences in single-stranded RNAs and snap-back RNAs from vesicular stomatitis defective interfering particles. J Gen Virol. 1978 Jan;38(1):35–50. doi: 10.1099/0022-1317-38-1-35. [DOI] [PubMed] [Google Scholar]
  22. Richardson C. C. Phosphorylation of nucleic acid by an enzyme from T4 bacteriophage-infected Escherichia coli. Proc Natl Acad Sci U S A. 1965 Jul;54(1):158–165. doi: 10.1073/pnas.54.1.158. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Schubert M., Keene J. D., Lazzarini R. A., Emerson S. U. The complete sequence of a unique RNA species synthesized by a DI particle of VSV. Cell. 1978 Sep;15(1):103–112. doi: 10.1016/0092-8674(78)90086-7. [DOI] [PubMed] [Google Scholar]
  24. Semler B. L., Perrault J., Abelson J., Holland J. J. Sequence of a RNA templated by the 3'-OH RNA terminus of defective interfering particles of vesicular stomatitis virus. Proc Natl Acad Sci U S A. 1978 Oct;75(10):4704–4708. doi: 10.1073/pnas.75.10.4704. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Simoncsits A., Brownlee G. G., Brown R. S., Rubin J. R., Guilley H. New rapid gel sequencing method for RNA. Nature. 1977 Oct 27;269(5631):833–836. doi: 10.1038/269833a0. [DOI] [PubMed] [Google Scholar]
  26. Wadsworth S., Hayward G. S., Roizman B. Anatomy of herpes simplex virus DNA. V. Terminally repetitive sequences. J Virol. 1976 Feb;17(2):503–512. doi: 10.1128/jvi.17.2.503-512.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Wolfson J., Dressler D. Adenovirus-2 DNA contains an inverted terminal repetition. Proc Natl Acad Sci U S A. 1972 Oct;69(10):3054–3057. doi: 10.1073/pnas.69.10.3054. [DOI] [PMC free article] [PubMed] [Google Scholar]

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