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. 1979 Sep 11;7(1):151–166. doi: 10.1093/nar/7.1.151

Formation of ribosome-RNA initiation complexes with alfalfa mosaic virus RNA 4 and RNA 3.

L Pinck, A Franck, C Fritsch
PMCID: PMC328002  PMID: 114984

Abstract

RNA 4 of alfalfa mosaic virus (AMV) is a monocistronic messenger for the coat protein. We have determined the sequence of the 40 +/- 2 nucleotides in RNA 4 that were protected in the initiation complex formed with wheat germ 80 S ribosomes from digestion by T1 or pancreatic ribonucleases. The AUG coat protein initiation codon was near the middle of this protected region. We have found two ribosome-binding sites in RNA 3. The principal one, near the 5' end, is the initiation site for the major translation product, a 35,000 dalton protein. The second site binds ribosomes only weakly, at the beginning of the "silent" coat protein cistron, and is similar but not identical to the initiation site protection site is discussed.

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  1. Dasgupta R., Shih D. S., Saris C., Kaesberg P. Nucleotide sequence of a viral RNA fragment that binds to eukaryotic ribosomes. Nature. 1975 Aug 21;256(5519):624–628. doi: 10.1038/256624a0. [DOI] [PubMed] [Google Scholar]
  2. Donis-Keller H., Maxam A. M., Gilbert W. Mapping adenines, guanines, and pyrimidines in RNA. Nucleic Acids Res. 1977 Aug;4(8):2527–2538. doi: 10.1093/nar/4.8.2527. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Efstratiadis A., Vournakis J. N., Donis-Keller H., Chaconas G., Dougall D. K., Kafatos F. C. End labeling of enzymatically decapped mRNA. Nucleic Acids Res. 1977 Dec;4(12):4165–4174. doi: 10.1093/nar/4.12.4165. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Frisby D. P., Newton C., Carey N. H., Fellner P., Newman J. F., Harris T. J., Brown F. Oligonucleotide mapping of picornavirus RNAs by two-dimensional electrophoresis. Virology. 1976 Jun;71(2):379–388. doi: 10.1016/0042-6822(76)90365-2. [DOI] [PubMed] [Google Scholar]
  5. Gould A. R., Symons R. H. Alfalfa mosaic virus RNA. Determination of the sequence homology between the four RNA species and a comparison with the four RNA species of cucumber mosaic virus. Eur J Biochem. 1978 Nov 2;91(1):269–278. doi: 10.1111/j.1432-1033.1978.tb20962.x. [DOI] [PubMed] [Google Scholar]
  6. Hagenbüchle O., Santer M., Steitz J. A., Mans R. J. Conservation of the primary structure at the 3' end of 18S rRNA from eucaryotic cells. Cell. 1978 Mar;13(3):551–563. doi: 10.1016/0092-8674(78)90328-8. [DOI] [PubMed] [Google Scholar]
  7. Jaspars E. M. Plant viruses with a multipartite genome. Adv Virus Res. 1974;19:37–149. doi: 10.1016/s0065-3527(08)60659-4. [DOI] [PubMed] [Google Scholar]
  8. Koper-Zwarthoff E. C., Lockard R. E., Alzner-deWeerd B., RajBhandary U. L., Bol J. F. Nucleotide sequence of 5' terminus of alfalfa mosaic virus RNA 4 leading into coat protein cistron. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5504–5508. doi: 10.1073/pnas.74.12.5504. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kozak M. How do eucaryotic ribosomes select initiation regions in messenger RNA? Cell. 1978 Dec;15(4):1109–1123. doi: 10.1016/0092-8674(78)90039-9. [DOI] [PubMed] [Google Scholar]
  10. Kozak M., Shatkin A. J. Characterization of ribosome-protected fragments from reovirus messenger RNA. J Biol Chem. 1976 Jul 25;251(14):4259–4266. [PubMed] [Google Scholar]
  11. Kozak M., Shatkin A. J. Identification of features in 5' terminal fragments from reovirus mRNA which are important for ribosome binding. Cell. 1978 Jan;13(1):201–212. doi: 10.1016/0092-8674(78)90150-2. [DOI] [PubMed] [Google Scholar]
  12. Marcu K., Dudock B. Characterization of a highly efficient protein synthesizing system derived from commercial wheat germ. Nucleic Acids Res. 1974 Nov;1(11):1385–1397. doi: 10.1093/nar/1.11.1385. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Mayo M. A., Fritsch C., Hirth L. Translation of tobacco rattle virus RNA in vitro using wheat germ extracts. Virology. 1976 Feb;69(2):408–415. doi: 10.1016/0042-6822(76)90472-4. [DOI] [PubMed] [Google Scholar]
  14. Mohier E., Hirth L., Le Meur M. A., Gerlinger P. Translation of alfalfa mosaic virus RNA's in mammalian cell-free systems. Virology. 1975 Dec;68(2):349–359. doi: 10.1016/0042-6822(75)90278-0. [DOI] [PubMed] [Google Scholar]
  15. Pelham H. R. Translation of fragmented viral RNA in vitro: initiation at multiple sites. FEBS Lett. 1979 Apr 1;100(1):195–199. doi: 10.1016/0014-5793(79)81162-x. [DOI] [PubMed] [Google Scholar]
  16. Pinck L. Blocked 5' termini in alfalfa mosaic virus RNA. Ann Microbiol (Paris) 1976 Jan;127A(1):175–181. [PubMed] [Google Scholar]
  17. Pinck L., Fauquet C. Analysis of the pancreatic-ribonuclease-digestion products of alfalfa-mosaic-virus ribonucleic acid. Sequence homologies between the different RNAs. Eur J Biochem. 1975 Sep 15;57(2):441–451. doi: 10.1111/j.1432-1033.1975.tb02319.x. [DOI] [PubMed] [Google Scholar]
  18. Pinck L., Hirth L. The replicative RNA and the viral RNA synthesis rate in tobacco infected with alfalfa mosaic virus. Virology. 1972 Aug;49(2):413–425. doi: 10.1016/0042-6822(72)90494-1. [DOI] [PubMed] [Google Scholar]
  19. Richards K., Guilley H., Jonard G., Hirth L. Nucleotide sequence at the 5' extremity of tobacco-mosaic-virus RNA. 1. The noncoding region (nucleotides 1-68). Eur J Biochem. 1978 Mar 15;84(2):513–519. doi: 10.1111/j.1432-1033.1978.tb12194.x. [DOI] [PubMed] [Google Scholar]
  20. Richardson C. C. Phosphorylation of nucleic acid by an enzyme from T4 bacteriophage-infected Escherichia coli. Proc Natl Acad Sci U S A. 1965 Jul;54(1):158–165. doi: 10.1073/pnas.54.1.158. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Rose J. K. Complete sequences of the ribosome recognition sites in vesicular stomatitis virus mRNAs: recognition by the 40S and 80S complexes. Cell. 1978 Jun;14(2):345–353. doi: 10.1016/0092-8674(78)90120-4. [DOI] [PubMed] [Google Scholar]
  22. Sanger F., Brownlee G. G., Barrell B. G. A two-dimensional fractionation procedure for radioactive nucleotides. J Mol Biol. 1965 Sep;13(2):373–398. doi: 10.1016/s0022-2836(65)80104-8. [DOI] [PubMed] [Google Scholar]
  23. Shih D. S., Kaesberg P. Translation of the RNAs of brome mosaic virus: the monocistronic nature of RNA1 and RNA2. J Mol Biol. 1976 May 5;103(1):77–88. doi: 10.1016/0022-2836(76)90053-x. [DOI] [PubMed] [Google Scholar]
  24. Shih D. S., Lane L. C., Kaesberg P. Origin of the small component of brome mosaic virus RNA. J Mol Biol. 1972 Mar 14;64(2):353–362. doi: 10.1016/0022-2836(72)90503-7. [DOI] [PubMed] [Google Scholar]
  25. Silberklang M., Gillum A. M., RajBhandary U. L. The use of nuclease P1 in sequence analysis of end group labeled RNA. Nucleic Acids Res. 1977 Dec;4(12):4091–4108. doi: 10.1093/nar/4.12.4091. [DOI] [PMC free article] [PubMed] [Google Scholar]

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