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. 1979 Sep 25;7(2):347–359. doi: 10.1093/nar/7.2.347

The distribution of histone H1 subfractions in chromatin subunits.

C Gorka, J J Lawrence
PMCID: PMC328022  PMID: 493149

Abstract

Rat liver chromatin was digested with micrococcal nuclease to various extents and fractionated into nucleosomes, di and trimers of nucleosomes on an isokinetic sucrose gradient. In conditions under which degradation of linker DNA within the particles was limited, the electrophoretic analysis of the histone content showed that the overall content of H1 histone increased from nucleosomes to higher order oligomers. Moreover, the histone H1 subfractions were found unevenly distributed among the chromatin subunits, one of them, H1--3 showing most variation. A more regular distribution of these subfractions was found in subunits obtained from a more extended digestion level of chromatin. It is suggested that the H1 subfractions differ in the protection they confer upon DNA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anachkova B., Russev G. Evidence for the existence of a fraction of rat liver chromatin containing only a few specific nonhistone proteins and reduced amount of histone H1. FEBS Lett. 1977 Sep 1;81(1):37–38. doi: 10.1016/0014-5793(77)80922-8. [DOI] [PubMed] [Google Scholar]
  2. Bakayev V. V., Bakayeva T. G., Varshavsky A. J. Nucleosomes and subnucleosomes: heterogeneity and composition. Cell. 1977 Jul;11(3):619–629. doi: 10.1016/0092-8674(77)90079-4. [DOI] [PubMed] [Google Scholar]
  3. Bellard M., Oudet P., Germond J. E., Chambon P. Subunit structure of simian-virus-40 minichromosome. Eur J Biochem. 1976 Nov 15;70(2):543–553. doi: 10.1111/j.1432-1033.1976.tb11046.x. [DOI] [PubMed] [Google Scholar]
  4. Bustin M., Stollar B. D. Immunochemical specificity in lysine-rich histone subfractions. J Biol Chem. 1972 Sep 25;247(18):5716–5721. [PubMed] [Google Scholar]
  5. Bustin M., Stollar B. D. Immunological relatedness of thymus and liver F1 histone subfracions. J Biol Chem. 1973 May 25;248(10):3506–3510. [PubMed] [Google Scholar]
  6. Chan P. K., Liew C. C. Identification of specific phosphoproteins in nuclease-digested chromatin subunits. Can J Biochem. 1977 Aug;55(8):847–855. doi: 10.1139/o77-125. [DOI] [PubMed] [Google Scholar]
  7. Gaubatz J. W., Chalkley R. Distribution of H1 histone in chromatin digested by micrococcal nuclease. Nucleic Acids Res. 1977 Oct;4(10):3281–3301. doi: 10.1093/nar/4.10.3281. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Goodwin G. H., Johns E. W. Are the high mobility group non-histone chromosomal proteins associated with 'active' chromatin? Biochim Biophys Acta. 1978 Jun 22;519(1):279–284. doi: 10.1016/0005-2787(78)90081-3. [DOI] [PubMed] [Google Scholar]
  9. Hartman P. G., Chapman G. E., Moss T., Bradbury E. M. Studies on the role and mode of operation of the very-lysine-rich histone H1 in eukaryote chromatin. The three structural regions of the histone H1 molecule. Eur J Biochem. 1977 Jul 1;77(1):45–51. doi: 10.1111/j.1432-1033.1977.tb11639.x. [DOI] [PubMed] [Google Scholar]
  10. Hewish D. R., Burgoyne L. A. Chromatin sub-structure. The digestion of chromatin DNA at regularly spaced sites by a nuclear deoxyribonuclease. Biochem Biophys Res Commun. 1973 May 15;52(2):504–510. doi: 10.1016/0006-291x(73)90740-7. [DOI] [PubMed] [Google Scholar]
  11. Kinkade J. M., Jr Qualitative species differences and quantitative tissue differences in the distribution of lysine-rich histones. J Biol Chem. 1969 Jun 25;244(12):3375–3386. [PubMed] [Google Scholar]
  12. Kornberg R. D. Chromatin structure: a repeating unit of histones and DNA. Science. 1974 May 24;184(4139):868–871. doi: 10.1126/science.184.4139.868. [DOI] [PubMed] [Google Scholar]
  13. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  14. Levy-Wilson B., Dixon G. H. Limited action of micrococcal nuclease on trout testis nuclei generates two mononucleosome subsets enriched in transcribed DNA sequences. Proc Natl Acad Sci U S A. 1979 Apr;76(4):1682–1686. doi: 10.1073/pnas.76.4.1682. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Noll M., Kornberg R. D. Action of micrococcal nuclease on chromatin and the location of histone H1. J Mol Biol. 1977 Jan 25;109(3):393–404. doi: 10.1016/s0022-2836(77)80019-3. [DOI] [PubMed] [Google Scholar]
  16. Noll M. Subunit structure of chromatin. Nature. 1974 Sep 20;251(5472):249–251. doi: 10.1038/251249a0. [DOI] [PubMed] [Google Scholar]
  17. Noll M., Thomas J. O., Kornberg R. D. Preparation of native chromatin and damage caused by shearing. Science. 1975 Mar 28;187(4182):1203–1206. doi: 10.1126/science.187.4182.1203. [DOI] [PubMed] [Google Scholar]
  18. Oudet P., Gross-Bellard M., Chambon P. Electron microscopic and biochemical evidence that chromatin structure is a repeating unit. Cell. 1975 Apr;4(4):281–300. doi: 10.1016/0092-8674(75)90149-x. [DOI] [PubMed] [Google Scholar]
  19. Prunell A., Kornberg R. D. Relation of nucleosomes to DNA sequences. Cold Spring Harb Symp Quant Biol. 1978;42(Pt 1):103–108. doi: 10.1101/sqb.1978.042.01.011. [DOI] [PubMed] [Google Scholar]
  20. Rall S. C., Cole R. D. Amino acid sequence and sequence variability of the amino-terminal regions of lysine-rich histones. J Biol Chem. 1971 Dec 10;246(23):7175–7190. [PubMed] [Google Scholar]
  21. Rall S. C., Okinaka R. T., Strniste G. F. Histone composition of nucleosomes isolated from cultured Chinese hamster cells. Biochemistry. 1977 Nov 1;16(22):4940–4944. doi: 10.1021/bi00641a031. [DOI] [PubMed] [Google Scholar]
  22. Renz M., Nehls P., Hozier J. Involvement of histone H1 in the organization of the chromosome fiber. Proc Natl Acad Sci U S A. 1977 May;74(5):1879–1883. doi: 10.1073/pnas.74.5.1879. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Shaw B. R., Herman T. M., Kovacic R. T., Beaudreau G. S., Van Holde K. E. Analysis of subunit organization in chicken erythrocyte chromatin. Proc Natl Acad Sci U S A. 1976 Feb;73(2):505–509. doi: 10.1073/pnas.73.2.505. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Singer D. S., Singer M. F. Studies on the interaction of H1 histone with superhelical DNA: characterization of the recognition and binding regions of H1 histones. Nucleic Acids Res. 1976 Oct;3(10):2531–2547. doi: 10.1093/nar/3.10.2531. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Thomas J. O., Kornberg R. D. An octamer of histones in chromatin and free in solution. Proc Natl Acad Sci U S A. 1975 Jul;72(7):2626–2630. doi: 10.1073/pnas.72.7.2626. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Varshavsky A. J., Bakayev V. V., Georgiev G. P. Heterogeneity of chromatin subunits in vitro and location of histone H1. Nucleic Acids Res. 1976 Feb;3(2):477–492. doi: 10.1093/nar/3.2.477. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Vidali G., Boffa L. C., Allfrey V. G. Selective release of chromosomal proteins during limited DNAase 1 digestion of avian erythrocyte chromatin. Cell. 1977 Oct;12(2):409–415. doi: 10.1016/0092-8674(77)90117-9. [DOI] [PubMed] [Google Scholar]
  28. Whitlock J. P., Jr, Simpson R. T. Removal of histone H1 exposes a fifty base pair DNA segment between nucleosomes. Biochemistry. 1976 Jul 27;15(15):3307–3314. doi: 10.1021/bi00660a022. [DOI] [PubMed] [Google Scholar]

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