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. 1979 Sep 25;7(2):481–492. doi: 10.1093/nar/7.2.481

Conformation of DNA in chromatin core particles containing poly(dAdT)-poly(dAdT) studied by 31 P NMR spectroscopy.

R T Simpson, H Shindo
PMCID: PMC328030  PMID: 493153

Abstract

We have prepared semi-synthetic chromatin core particles from a complex of chicken erythrocyte inner histones (H2A, H2B, H3 and H4) with double-stranded poly(dAdT).poly(dAdT) and studied the conformation of the phosphodiester backbone using 31P NMR at 109.3 MHz. At 20 degrees C, the core particle spectrum is fit well by a single Lorenzian distribution with a line width of 110 Hz. This signal is significantly broader than that for the 145 base pair poly(dAdT).poly(dAdT) alone; the latter consists of two resonances, approximately equal in intensity, with average line width 41 Hz. Major changes in the spectrum ensue on heating the core particle preparation. In conjunction with other results (1) these data suggest four states for the core particle at increasing temperatures. Additionally, analysis of the spectrum of the unmelted core particle and its differences from protein-free DNA of the same length suggests that the conformation of the phosphodiester backbone and/or its interactions with histones along the length of the core particle DNA segment may not be uniform.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cotter R. I., Lilley D. M. The conformation of DNA and protein within chromatin subunits. FEBS Lett. 1977 Oct 1;82(1):63–68. doi: 10.1016/0014-5793(77)80886-7. [DOI] [PubMed] [Google Scholar]
  2. Crick F. H., Klug A. Kinky helix. Nature. 1975 Jun 12;255(5509):530–533. doi: 10.1038/255530a0. [DOI] [PubMed] [Google Scholar]
  3. Kallenbach N. R., Appleby D. W., Bradley C. H. 31P magnetic resonance of DNA in nucleosome core particles of chromatin. Nature. 1978 Mar 9;272(5649):134–138. doi: 10.1038/272134a0. [DOI] [PubMed] [Google Scholar]
  4. Klevan L., Armitage I. M., Crothers D. M. 31P NMR studies of the solution structure and dynamics of nucleosomes and DNA. Nucleic Acids Res. 1979 Apr;6(4):1607–1616. doi: 10.1093/nar/6.4.1607. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Simpson R. T., Künzler P. Cromatin and core particles formed from the inner histones and synthetic polydeoxyribonucleotides of defined sequence. Nucleic Acids Res. 1979 Apr;6(4):1387–1415. doi: 10.1093/nar/6.4.1387. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Sobell H. M., Tsai C. C., Gilbert S. G., Jain S. C., Sakore T. D. Organization of DNA in chromatin. Proc Natl Acad Sci U S A. 1976 Sep;73(9):3068–3072. doi: 10.1073/pnas.73.9.3068. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Weischet W. O., Tatchell K., Van Holde K. E., Klump H. Thermal denaturation of nucleosomal core particles. Nucleic Acids Res. 1978 Jan;5(1):139–160. doi: 10.1093/nar/5.1.139. [DOI] [PMC free article] [PubMed] [Google Scholar]

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