Skip to main content
PMC home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1979 Sep 25;7(2):493–500. doi: 10.1093/nar/7.2.493

Sequence homology adjacent to the 3' terminal poly(A) of cowpea mosaic virus RNAs.

J W Davies, J Stanley, A Van Kammen
PMCID: PMC328031  PMID: 91160

Abstract

We have determined the sequence of 80 nucleotides adjacent to 3' poly(A) of both (middle and bottom component) cowpea mosaic virus RNAs, using dideoxynucleotide termination of reverse transcription. Sequence conservation is indicated, there being about 80% homology between the first 65 bases of each RNA. Although both RNAs are polyadenylated, there is no AAUAAA sequence as associated with most known polyadenylated mRNAs of eukaryotes and viruses. However both RNAs are U and A-rich in this region.

Full text

PDF
493

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bastin M., Dasgupta R., Hall T. C., Kaesberg P. Similarity in structure and function of the 3'-terminal region of the four brome mosaic viral RNAs. J Mol Biol. 1976 Jun 5;103(4):737–745. doi: 10.1016/0022-2836(76)90206-0. [DOI] [PubMed] [Google Scholar]
  2. Briand J. P., Jonard G., Guilley H., Richards K., Hirth L. Nucleotide sequence (n=159) of the amino-acid-accepting 3'-OH extremity of turnip-yellow-mosaic-virus RNA and the last portion of its coat-protein cistron. Eur J Biochem. 1977 Feb;72(3):453–463. doi: 10.1111/j.1432-1033.1977.tb11269.x. [DOI] [PubMed] [Google Scholar]
  3. Daubert S. D., Bruening G., Najarian R. C. Protein bound to the genome RNAs of cowpea mosaic virus. Eur J Biochem. 1978 Dec 1;92(1):45–51. doi: 10.1111/j.1432-1033.1978.tb12721.x. [DOI] [PubMed] [Google Scholar]
  4. Davies J. W., Aalbers A. M., Stuik E. J., Van Kammen A. Translation of cowpea mosaic virus RNA in a cell-free extract from wheat germ. FEBS Lett. 1977 May 15;77(2):265–269. doi: 10.1016/0014-5793(77)80248-2. [DOI] [PubMed] [Google Scholar]
  5. Davies J. W., Verver J. W., Goldbach R. W., Van Kammen A. Efficient reverse transcription of cowpea mosaic virus RNAs. Nucleic Acids Res. 1978 Dec;5(12):4643–4661. doi: 10.1093/nar/5.12.4643. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. De Jager C. P. Genetic analysis of cowpea mosaic virus mutants by supplementation and reassortment tests. Virology. 1976 Mar;70(1):151–163. doi: 10.1016/0042-6822(76)90245-2. [DOI] [PubMed] [Google Scholar]
  7. Devos R., Van Emmelo J., Celen P., Gillis E., Fiers W. Synthesis by avian-myeloblastosis-virus RNA-dependent DNA polymerase of discrete reverse transcripts of bacteriophage RNA polyadenylated in vitro. Eur J Biochem. 1977 Oct 3;79(2):419–432. doi: 10.1111/j.1432-1033.1977.tb11824.x. [DOI] [PubMed] [Google Scholar]
  8. Flanegan J. B., Petterson R. F., Ambros V., Hewlett N. J., Baltimore D. Covalent linkage of a protein to a defined nucleotide sequence at the 5'-terminus of virion and replicative intermediate RNAs of poliovirus. Proc Natl Acad Sci U S A. 1977 Mar;74(3):961–965. doi: 10.1073/pnas.74.3.961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hamlyn P. H., Browniee G. G., Cheng C. C., Gait M. J., Milstein C. Complete sequence of constant and 3' noncoding regions of an immunoglobulin mRNA using the dideoxynucleotide method of RNA sequencing. Cell. 1978 Nov;15(3):1067–1075. doi: 10.1016/0092-8674(78)90290-8. [DOI] [PubMed] [Google Scholar]
  10. Lamy D., Jonard G., Guilley H., Hirth L. Comparison between the 3'OH end RNA sequence of two strains of tobacco mosaic virus (TMV) which may be aminoacylated. FEBS Lett. 1975 Dec 1;60(1):202–204. doi: 10.1016/0014-5793(75)80451-0. [DOI] [PubMed] [Google Scholar]
  11. McGeoch D. J., Turnbull N. T. Analysis of the 3'-terminal nucleotide sequence of vesicular stomatitis virus N protein mRNA. Nucleic Acids Res. 1978 Nov;5(11):4007–4024. doi: 10.1093/nar/5.11.4007. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Nomoto A., Detjen B., Pozzatti R., Wimmer E. The location of the polio genome protein in viral RNAs and its implication for RNA synthesis. Nature. 1977 Jul 21;268(5617):208–213. doi: 10.1038/268208a0. [DOI] [PubMed] [Google Scholar]
  13. Porter A. G., Fellner P., Black D. N., Rowlands D. J., Harris T. J., Brown F. 3'-Terminal nucleotide sequences in the genome RNA of picornaviruses. Nature. 1978 Nov 16;276(5685):298–301. doi: 10.1038/276298a0. [DOI] [PubMed] [Google Scholar]
  14. Porter A. G., Merregaert J., Van Emmelo J., Fiers W. Sequence of 129 nucleotides at the 3'-terminus of encephalomyocarditis virus RNA. Eur J Biochem. 1978 Jul 3;87(3):551–561. doi: 10.1111/j.1432-1033.1978.tb12406.x. [DOI] [PubMed] [Google Scholar]
  15. Proudfoot N. J., Brownlee G. G. 3' non-coding region sequences in eukaryotic messenger RNA. Nature. 1976 Sep 16;263(5574):211–214. doi: 10.1038/263211a0. [DOI] [PubMed] [Google Scholar]
  16. Sanger F., Coulson A. R. The use of thin acrylamide gels for DNA sequencing. FEBS Lett. 1978 Mar 1;87(1):107–110. doi: 10.1016/0014-5793(78)80145-8. [DOI] [PubMed] [Google Scholar]
  17. Silberklang M., Gillum A. M., RajBhandary U. L. The use of nuclease P1 in sequence analysis of end group labeled RNA. Nucleic Acids Res. 1977 Dec;4(12):4091–4108. doi: 10.1093/nar/4.12.4091. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Stanley J., Rottier P., Davies J. W., Zabel P., Van Kammen A. A protein linked to the 5' termini of both RNA components of the cowpea mosaic virus genome. Nucleic Acids Res. 1978 Dec;5(12):4505–4522. doi: 10.1093/nar/5.12.4505. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Steele K. P., Frist R. H. Characterization of the 3' Termini of the RNAs of Cowpea Mosaic Virus. J Virol. 1978 May;26(2):243–248. doi: 10.1128/jvi.26.2.243-248.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Zimmern D., Kaesberg P. 3'-terminal nucleotide sequence of encephalomyocarditis virus RNA determined by reverse transcriptase and chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1978 Sep;75(9):4257–4261. doi: 10.1073/pnas.75.9.4257. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Zimmern D., Kaesberg P. 3'-terminal nucleotide sequence of encephalomyocarditis virus RNA determined by reverse transcriptase and chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1978 Sep;75(9):4257–4261. doi: 10.1073/pnas.75.9.4257. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. el-Manna M. M., Bruening G. Polyadenylate sequences in the ribonucleic acids of cowpea mosaic virus. Virology. 1973 Nov;56(1):198–206. doi: 10.1016/0042-6822(73)90299-7. [DOI] [PubMed] [Google Scholar]
  23. van Kammen A. The relationship between the components of cowpea mosaic virus. I. Two ribonucleoprotein particles necessary for the infectivity of CPMV. Virology. 1968 Feb;34(2):312–318. doi: 10.1016/0042-6822(68)90241-9. [DOI] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES