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Subcellular localization of Cda2 in C. neoformans. (A) Western blot analysis of fractions collected from wild-type serotype A strain KN99 and a cda2Δ strain in the same background probed with monoclonal antibodies directed against a Cda2-specific peptide. Cy, cytosol; S, secretions; CW, cell wall; M, membrane. (B) Control blots containing fractions from KN99 demonstrating the presence of actin in the cytosolic fraction and it absence from the remaining fractions.
