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. 1979 Oct 10;7(3):669–677. doi: 10.1093/nar/7.3.669

The polarity of the cauliflower mosaic virus genome.

R Hull, S N Covey, J Stanley, J W Davies
PMCID: PMC328046  PMID: 503842

Abstract

We have demonstrated that the 5' termini of the DNAs of cauliflower mosaic virus (CaMV) isolates CM4-184 and Cabb B-JI have free hydroxyl groups accessible for phosphorylation using polynucleotide kinase. This has enabled us, using the Cabb B-JI isolate, to label and identify the 5' nucleotides. Digestion of 5' terminally labelled DNA with EcoRI restriction endonuclease revealed that only three of the eleven single-stranded EcoRI fragments were labelled, those situated adjacent to the three known gaps in the double-stranded genome. Identification of the labelled fragments indicated the polarity of the genome. Hybridization experiments using RNA transcripts from infected leaves gave results consistent with the polarity data and confirmed the direction of transcription. Transcription on the largest DNA strand (alpha strand) is initiated between map units 0.24 and 0.30 and terminates between map units 0.0 and 0.24.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Covey S. N., Grierson D. Subcellular distribution and properties of poly(A)-containing RNA from cultured plant cells. Eur J Biochem. 1976 Apr 1;63(2):599–606. doi: 10.1111/j.1432-1033.1976.tb10264.x. [DOI] [PubMed] [Google Scholar]
  2. Finkelstein M., Rownd T. H. A rapid method for extracting DNA from agarose gels. Plasmid. 1978 Sep;1(4):557–562. doi: 10.1016/0147-619x(78)90012-4. [DOI] [PubMed] [Google Scholar]
  3. Howell S. H., Hull R. Replication of cauliflower mosaic virus and transcription of its genome in turnip leaf protoplasts. Virology. 1978 May 15;86(2):468–481. doi: 10.1016/0042-6822(78)90086-7. [DOI] [PubMed] [Google Scholar]
  4. Hull R., Howell S. H. Structure of the cauliflower mosaic virus genome. II. Variation in DNA structure and sequence between isolates. Virology. 1978 May 15;86(2):482–493. doi: 10.1016/0042-6822(78)90087-9. [DOI] [PubMed] [Google Scholar]
  5. Lev Z., Kamen R., Manor H. Topography of polyoma virus-specific giant nuclear RNA molecules containing poly(A) sequences. Virology. 1979 Mar;93(2):445–457. doi: 10.1016/0042-6822(79)90248-4. [DOI] [PubMed] [Google Scholar]
  6. Maxam A. M., Gilbert W. A new method for sequencing DNA. Proc Natl Acad Sci U S A. 1977 Feb;74(2):560–564. doi: 10.1073/pnas.74.2.560. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. McDonell M. W., Simon M. N., Studier F. W. Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels. J Mol Biol. 1977 Feb 15;110(1):119–146. doi: 10.1016/s0022-2836(77)80102-2. [DOI] [PubMed] [Google Scholar]
  8. Shepherd R. J. DNA viruses of higher plants. Adv Virus Res. 1976;20:305–339. doi: 10.1016/s0065-3527(08)60508-4. [DOI] [PubMed] [Google Scholar]
  9. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  10. Volovitch M., Drugeon C., Yot P. Studies on the single-stranded discontinuities of the cauliflower mosaic virus genome. Nucleic Acids Res. 1978 Aug;5(8):2913–2925. doi: 10.1093/nar/5.8.2913. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Weiss B., Live T. R., Richardson C. C. Enzymatic breakage and joining of deoxyribonucleic acid. V. End group labeling and analysis of deoxyribonucleic acid containing single straned breaks. J Biol Chem. 1968 Sep 10;243(17):4530–4542. [PubMed] [Google Scholar]

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