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. 1980 Dec 20;8(24):6059–6068. doi: 10.1093/nar/8.24.6059

Multiple phases of nucleosomes in the hsp 70 genes of Drosophila melanogaster.

A Levy, M Noll
PMCID: PMC328072  PMID: 6258161

Abstract

The arrangement of the nucleosomes with respect to the DNA sequence has been examined in the genes coding for the major heat shock protein (hsp 70) in Drosophila. In the repressed state of the genes, the nucleosomes are precisely phased in at least three frames.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Artavanis-Tsakonas S., Schedl P., Mirault M. E., Moran L., Lis J. Genes for the 70,000 dalton heat shock protein in two cloned D. melanogaster DNA segments. Cell. 1979 May;17(1):9–18. doi: 10.1016/0092-8674(79)90290-3. [DOI] [PubMed] [Google Scholar]
  2. Chao M. V., Gralla J., Martinson H. G. DNA sequence directs placement of histone cores on restriction fragments during nucleosome formation. Biochemistry. 1979 Mar 20;18(6):1068–1074. doi: 10.1021/bi00573a021. [DOI] [PubMed] [Google Scholar]
  3. Crémisi C., Pignatti P. F., Yaniv M. Random location and absence of movement of the nucleosomes on SV 40 nucleoprotein complex isolated from infected cells. Biochem Biophys Res Commun. 1976 Dec 6;73(3):548–554. doi: 10.1016/0006-291x(76)90845-7. [DOI] [PubMed] [Google Scholar]
  4. Ermini M., Kuenzle C. C. The chromatin repeat length of cortical neurons shortens during early posnatal development. FEBS Lett. 1978 Jun 1;90(1):167–172. doi: 10.1016/0014-5793(78)80322-6. [DOI] [PubMed] [Google Scholar]
  5. Fittler F., Zachau H. G. Subunit structure of alpha-satellite DNA containing chromatin from African green monkey cells. Nucleic Acids Res. 1979 Sep 11;7(1):1–13. doi: 10.1093/nar/7.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Ingolia T. D., Craig E. A., McCarthy B. J. Sequence of three copies of the gene for the major Drosophila heat shock induced protein and their flanking regions. Cell. 1980 Oct;21(3):669–679. doi: 10.1016/0092-8674(80)90430-4. [DOI] [PubMed] [Google Scholar]
  7. Ish-Horowicz D., Pinchin S. M., Schedl P., Artavanis-Tsakonas S., Mirault M. E. Genetic and molecular analysis of the 87A7 and 87C1 heat-inducible loci of D. melanogaster. Cell. 1979 Dec;18(4):1351–1358. doi: 10.1016/0092-8674(79)90245-9. [DOI] [PubMed] [Google Scholar]
  8. Kornberg R. D. Chromatin structure: a repeating unit of histones and DNA. Science. 1974 May 24;184(4139):868–871. doi: 10.1126/science.184.4139.868. [DOI] [PubMed] [Google Scholar]
  9. Kornberg R. D. Structure of chromatin. Annu Rev Biochem. 1977;46:931–954. doi: 10.1146/annurev.bi.46.070177.004435. [DOI] [PubMed] [Google Scholar]
  10. Levy A., Frei E., Noll M. Efficient transfer of highly resolved small DNA fragments from polyacrylamide gels to DBM paper. Gene. 1980 Nov;11(3-4):283–290. doi: 10.1016/0378-1119(80)90068-2. [DOI] [PubMed] [Google Scholar]
  11. Levy A., Jakob K. M. Nascent DNA in nucleosome like structures from chromatin. Cell. 1978 Jun;14(2):259–267. doi: 10.1016/0092-8674(78)90112-5. [DOI] [PubMed] [Google Scholar]
  12. Maniatis T., Jeffrey A., Kleid D. G. Nucleotide sequence of the rightward operator of phage lambda. Proc Natl Acad Sci U S A. 1975 Mar;72(3):1184–1188. doi: 10.1073/pnas.72.3.1184. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Maniatis T., Jeffrey A., van deSande H. Chain length determination of small double- and single-stranded DNA molecules by polyacrylamide gel electrophoresis. Biochemistry. 1975 Aug 26;14(17):3787–3794. doi: 10.1021/bi00688a010. [DOI] [PubMed] [Google Scholar]
  14. McGhee J. D., Felsenfeld G. Nucleosome structure. Annu Rev Biochem. 1980;49:1115–1156. doi: 10.1146/annurev.bi.49.070180.005343. [DOI] [PubMed] [Google Scholar]
  15. Mirault M. E., Goldschmidt-Clermont M., Artavanis-Tsakonas S., Schedl P. Organization of the multiple genes for the 70,000-dalton heat-shock protein in Drosophila melanogaster. Proc Natl Acad Sci U S A. 1979 Oct;76(10):5254–5258. doi: 10.1073/pnas.76.10.5254. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Morris N. R. A comparison of the structure of chicken erythrocyte and chicken liver chromatin. Cell. 1976 Dec;9(4 Pt 1):627–632. doi: 10.1016/0092-8674(76)90045-3. [DOI] [PubMed] [Google Scholar]
  17. Musich P. R., Maio J. J., Brown F. L. Subunit structure of chromatin and the organization of eukaryotic highly repetitive DNA: indications of a phase relation between restriction sites and chromatin subunits in African green monkey and calf nuclei. J Mol Biol. 1977 Dec 15;117(3):657–677. doi: 10.1016/0022-2836(77)90063-8. [DOI] [PubMed] [Google Scholar]
  18. Noll M. DNA folding in the nucleosome. J Mol Biol. 1977 Oct 15;116(1):49–71. doi: 10.1016/0022-2836(77)90118-8. [DOI] [PubMed] [Google Scholar]
  19. Noll M., Kornberg R. D. Action of micrococcal nuclease on chromatin and the location of histone H1. J Mol Biol. 1977 Jan 25;109(3):393–404. doi: 10.1016/s0022-2836(77)80019-3. [DOI] [PubMed] [Google Scholar]
  20. Polisky B., McCarthy B. Location of histones on simian virus 40 DNA. Proc Natl Acad Sci U S A. 1975 Aug;72(8):2895–2899. doi: 10.1073/pnas.72.8.2895. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Ponder B. A., Crawford L. V. The arrangement of nucleosomes in nucleoprotein complexes from polyoma virus and SV40. Cell. 1977 May;11(1):35–49. doi: 10.1016/0092-8674(77)90315-4. [DOI] [PubMed] [Google Scholar]
  22. Prunell A., Kornberg R. D., Lutter L., Klug A., Levitt M., Crick F. H. Periodicity of deoxyribonuclease I digestion of chromatin. Science. 1979 May 25;204(4395):855–858. doi: 10.1126/science.441739. [DOI] [PubMed] [Google Scholar]
  23. Prunell A., Kornberg R. D. Relation of nucleosomes to DNA sequences. Cold Spring Harb Symp Quant Biol. 1978;42(Pt 1):103–108. doi: 10.1101/sqb.1978.042.01.011. [DOI] [PubMed] [Google Scholar]
  24. Prunell A., Kornberg R. D. Relation of nucleosomes to nucleotide sequences in the rat. Philos Trans R Soc Lond B Biol Sci. 1978 May 11;283(997):269–273. doi: 10.1098/rstb.1978.0023. [DOI] [PubMed] [Google Scholar]
  25. ROBERTS W. K., DEKKER C. A., RUSHIZKY G. W., KNIGHT C. A. Studies on the mechanism of action of micrococcal nuclease. 1. Degradation of thymus deoxyribonucleic acid. Biochim Biophys Acta. 1962 May 14;55:664–673. doi: 10.1016/0006-3002(62)90844-2. [DOI] [PubMed] [Google Scholar]
  26. Shaw B. R., Herman T. M., Kovacic R. T., Beaudreau G. S., Van Holde K. E. Analysis of subunit organization in chicken erythrocyte chromatin. Proc Natl Acad Sci U S A. 1976 Feb;73(2):505–509. doi: 10.1073/pnas.73.2.505. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Spadafora C., Bellard M., Compton J. L., Chambon P. The DNA repeat lengths in chromatins from sea urchin sperm and gastrule cells are markedly different. FEBS Lett. 1976 Oct 15;69(1):281–285. doi: 10.1016/0014-5793(76)80704-1. [DOI] [PubMed] [Google Scholar]
  28. Sutcliffe J. G. pBR322 restriction map derived from the DNA sequence: accurate DNA size markers up to 4361 nucleotide pairs long. Nucleic Acids Res. 1978 Aug;5(8):2721–2728. doi: 10.1093/nar/5.8.2721. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Thomas J. O., Thompson R. J. Variation in chromatin structure in two cell types from the same tissue: a short DNA repeat length in cerebral cortex neurons. Cell. 1977 Apr;10(4):633–640. doi: 10.1016/0092-8674(77)90096-4. [DOI] [PubMed] [Google Scholar]
  30. Török I., Karch F. Nucleotide sequences of heat shock activated genes in Drosophila melanogaster. I. Sequences in the regions of the 5' and 3' ends of the hsp 70 gene in the hybrid plasmid 56H8. Nucleic Acids Res. 1980 Jul 25;8(14):3105–3123. doi: 10.1093/nar/8.14.3105. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Wittig B., Wittig S. A phase relationship associates tRNA structural gene sequences with nucleosome cores. Cell. 1979 Dec;18(4):1173–1183. doi: 10.1016/0092-8674(79)90230-7. [DOI] [PubMed] [Google Scholar]
  32. Wu C. The 5' ends of Drosophila heat shock genes in chromatin are hypersensitive to DNase I. Nature. 1980 Aug 28;286(5776):854–860. doi: 10.1038/286854a0. [DOI] [PubMed] [Google Scholar]
  33. Wu C., Wong Y. C., Elgin S. C. The chromatin structure of specific genes: II. Disruption of chromatin structure during gene activity. Cell. 1979 Apr;16(4):807–814. doi: 10.1016/0092-8674(79)90096-5. [DOI] [PubMed] [Google Scholar]

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