Skip to main content
NCBI home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1980 Dec 20;8(24):6069–6079. doi: 10.1093/nar/8.24.6069

Histone H1o: its location in chromatin.

B J Smith, E W Johns
PMCID: PMC328073  PMID: 7465429

Abstract

Histone H1o is an H5-like protein found in mammals. Its location in chromatin from animal tissues has been studied by micrococcal nuclease digestion and by quantitation. It was found that H1o occurs in the linker region of chromatin, and replaces H1 there as it does so. As much as a third of the H1 becomes replaced, at least in some tissues. The abundance of H1o was similar in bulk chromatin and in a mononucleosome population which was putatively enriched in transcribed DNA sequences. It was concluded that H1o probably does not suppress transcription.

Full text

PDF
6069

Images in this article

6072Image
on p.6072

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Albright S. C., Wiseman J. M., Lange R. A., Garrard W. T. Subunit structures of different electrophoretic forms of nucleosomes. J Biol Chem. 1980 Apr 25;255(8):3673–3684. [PubMed] [Google Scholar]
  2. Bakayev V. V., Bakayeva T. G., Varshavsky A. J. Nucleosomes and subnucleosomes: heterogeneity and composition. Cell. 1977 Jul;11(3):619–629. doi: 10.1016/0092-8674(77)90079-4. [DOI] [PubMed] [Google Scholar]
  3. Bakayeva T. G., Bakayev V. V. Separation of nucleosomes containing histones H1 and H5. Mol Biol Rep. 1978 Oct 16;4(3):185–189. doi: 10.1007/BF00777522. [DOI] [PubMed] [Google Scholar]
  4. Balhorn R., Chalkley R., Granner D. Lysine-rich histone phosphorylation. A positive correlation with cell replication. Biochemistry. 1972 Mar 14;11(6):1094–1098. doi: 10.1021/bi00756a023. [DOI] [PubMed] [Google Scholar]
  5. Bloom K. S., Anderson J. N. Fractionation of hen oviduct chromatin into transcriptionally active and inactive regions after selective micrococcal nuclease digestion. Cell. 1978 Sep;15(1):141–150. doi: 10.1016/0092-8674(78)90090-9. [DOI] [PubMed] [Google Scholar]
  6. Bradbury E. M., Crane-Robinson C., Johns E. W. Specific conformations and interactions in chicken erythrocyte histone F2C. Nat New Biol. 1972 Aug 30;238(87):262–264. doi: 10.1038/newbio238262a0. [DOI] [PubMed] [Google Scholar]
  7. Chikaraishi D. M., Deeb S. S., Sueoka N. Sequence complexity of nuclear RNAs in adult rat tissues. Cell. 1978 Jan;13(1):111–120. doi: 10.1016/0092-8674(78)90142-3. [DOI] [PubMed] [Google Scholar]
  8. D'Anna J. A., Tobey R. A., Gurley L. R. Concentration-dependent effects of sodium butyrate in Chinese hamster cells: cell-cycle progression, inner-histone acetylation, histone H1 dephosphorylation, and induction of an H1-like protein. Biochemistry. 1980 Jun 10;19(12):2656–2671. doi: 10.1021/bi00553a019. [DOI] [PubMed] [Google Scholar]
  9. Davie J. R., Candido E. P. Acetylated histone H4 is preferentially associated with template-active chromatin. Proc Natl Acad Sci U S A. 1978 Aug;75(8):3574–3577. doi: 10.1073/pnas.75.8.3574. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Detke S., Lichtler A., Phillips I., Stein J., Stein G. Reassessment of histone gene expression during cell cycle in human cells by using homologous H4 histone cDNA. Proc Natl Acad Sci U S A. 1979 Oct;76(10):4995–4999. doi: 10.1073/pnas.76.10.4995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Dick C., Johns E. W. A quantitative comparison of histones from immature and mature erythroid cells of the duck. Biochim Biophys Acta. 1969 Mar;175(2):414–418. doi: 10.1016/0005-2795(69)90020-8. [DOI] [PubMed] [Google Scholar]
  12. Elgin S. C., Weintraub H. Chromosomal proteins and chromatin structure. Annu Rev Biochem. 1975;44:725–774. doi: 10.1146/annurev.bi.44.070175.003453. [DOI] [PubMed] [Google Scholar]
  13. Hildebrand C. E., Walters R. A. Rapid assembly of newly synthesized DNA into chromatin subunits prior to joining to small DNA replication intermediates. Biochem Biophys Res Commun. 1976 Nov 8;73(1):157–163. doi: 10.1016/0006-291x(76)90510-6. [DOI] [PubMed] [Google Scholar]
  14. Keppel F., Allet B., Eisen H. Biochemical properties and localization of the chromosomal protein IP25. Eur J Biochem. 1979 Jun 1;96(3):477–482. doi: 10.1111/j.1432-1033.1979.tb13060.x. [DOI] [PubMed] [Google Scholar]
  15. Kuenzle C. C., Bregnard A., Hübscher U., Ruch F. Extra DNA in forebrain cortical neurons. Exp Cell Res. 1978 Apr;113(1):151–160. doi: 10.1016/0014-4827(78)90095-2. [DOI] [PubMed] [Google Scholar]
  16. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  17. Levy-Wilson B., Dixon G. H. Limited action of micrococcal nuclease on trout testis nuclei generates two mononucleosome subsets enriched in transcribed DNA sequences. Proc Natl Acad Sci U S A. 1979 Apr;76(4):1682–1686. doi: 10.1073/pnas.76.4.1682. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Marsh W. H., Fitzgerald P. J. Pancreas acinar cell regeneration. 13. Histone synthesis and modification. Fed Proc. 1973 Nov;32(11):2119–2125. [PubMed] [Google Scholar]
  19. Mazrimas J. A., Balhorn R., Hatch F. T. Separation of satellite DNA chromatin and main band DNA chromatin from mouse brain. Nucleic Acids Res. 1979 Oct 25;7(4):935–946. doi: 10.1093/nar/7.4.935. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. McGhee J. D., Felsenfeld G. Nucleosome structure. Annu Rev Biochem. 1980;49:1115–1156. doi: 10.1146/annurev.bi.49.070180.005343. [DOI] [PubMed] [Google Scholar]
  21. Medvedev Z. A., Medvedeva M. N., Huschtscha L. I. Age changes of the pattern of F1 histone subfractions in rat liver and spleen chromatin. Gerontology. 1977;23(5):334–341. doi: 10.1159/000212205. [DOI] [PubMed] [Google Scholar]
  22. Miki B. L., Neelin J. M. DNA repeat lengths of erythrocyte chromatins differing in content of histones H1 and H5. Nucleic Acids Res. 1980 Feb 11;8(3):529–542. doi: 10.1093/nar/8.3.529. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Miul'berg A. A., Tishchenko L. I., Karaiagina I. Iu, Grigorbeva M. B. Analiz subdedinichnoí organizatsiikhromatina mozga. Biokhimiia. 1978 Feb;43(2):340–349. [PubMed] [Google Scholar]
  24. Nadeau P., Oliver D. R., Chalkley R. Effect of inhibition of DNA synthesis on histone synthesis and deposition. Biochemistry. 1978 Nov 14;17(23):4885–4893. doi: 10.1021/bi00616a005. [DOI] [PubMed] [Google Scholar]
  25. Panyim S., Chalkley R. The heterogeneity of histones. I. A quantitative analysis of calf histones in very long polyacrylamide gels. Biochemistry. 1969 Oct;8(10):3972–3979. doi: 10.1021/bi00838a013. [DOI] [PubMed] [Google Scholar]
  26. Pehrson J., Cole R. D. Histone H10 accumulates in growth-inhibited cultured cells. Nature. 1980 May 1;285(5759):43–44. doi: 10.1038/285043a0. [DOI] [PubMed] [Google Scholar]
  27. Simpson R. T. Structure of the chromatosome, a chromatin particle containing 160 base pairs of DNA and all the histones. Biochemistry. 1978 Dec 12;17(25):5524–5531. doi: 10.1021/bi00618a030. [DOI] [PubMed] [Google Scholar]
  28. Smith B. J., Johns E. W. Isolation and characterisation of subfractions of nuclear protein H1 degree. FEBS Lett. 1980 Jan 28;110(1):25–29. doi: 10.1016/0014-5793(80)80014-7. [DOI] [PubMed] [Google Scholar]
  29. Smith B. J., Walker J. M., Johns E. W. Structural homology between a mammalian H1(0) subfraction and avian erythrocyte-specific histone H5. FEBS Lett. 1980 Mar 24;112(1):42–44. doi: 10.1016/0014-5793(80)80122-0. [DOI] [PubMed] [Google Scholar]
  30. Todd R. D., Garrard W. T. Two-dimensional electrophoretic analysis of polynucleosomes. J Biol Chem. 1977 Jul 10;252(13):4729–4738. [PubMed] [Google Scholar]
  31. Valkonen K. H., Piha R. S. Isoelectric focusing and isoelectric points of bovine liver histones. Anal Biochem. 1980 May 15;104(2):499–505. doi: 10.1016/0003-2697(80)90107-4. [DOI] [PubMed] [Google Scholar]
  32. Varley J. M., Macgregor H. C., Erba H. P. Satellite DNA is transcribed on lampbrush chromosomes. Nature. 1980 Feb 14;283(5748):686–688. doi: 10.1038/283686a0. [DOI] [PubMed] [Google Scholar]
  33. Varshavsky A. J., Bakayev V. V., Nedospasov S. A., Georgiev G. P. On the structure of eukaryotic, prokaryotic, and viral chromatin. Cold Spring Harb Symp Quant Biol. 1978;42(Pt 1):457–473. doi: 10.1101/sqb.1978.042.01.049. [DOI] [PubMed] [Google Scholar]
  34. Weintraub H. The nucleosome repeat length increases during erythropoiesis in the chick. Nucleic Acids Res. 1978 Apr;5(4):1179–1188. doi: 10.1093/nar/5.4.1179. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES