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. 1980 Dec 20;8(24):6221–6231. doi: 10.1093/nar/8.24.6221

The conformation of a large RNA fragment from the E.coli ribosomal 16S-RNA. An X-ray and neutron small-angle scattering study.

R Osterberg, B Sjöberg, G A Garrett, R Müller
PMCID: PMC328084  PMID: 6162161

Abstract

A large 12S RNA fragment which constitutes the 5' two-thirds of 16S-RNA from the E. coli 30S subunit has been investigated by small-angle X-ray and neutron scattering. The results indicate that in reconstitution buffer the 12S-RNA fragment has a molecular weight of 270,000 +/- 20,000 and a radius of gyration of 7.1 nm. The scattering data are compatible with the RNA being folded into two major domains with the shapes of two adjacent, quite similar cylinders.

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Selected References

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