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. 1991 Apr 25;19(8):1753–1758. doi: 10.1093/nar/19.8.1753

Structure and in vitro replication of DNA templates containing 7,8-dihydro-8-oxoadenine.

W Guschlbauer 1, A M Duplaa 1, A Guy 1, R Téoule 1, G V Fazakerley 1
PMCID: PMC328100  PMID: 1851559

Abstract

Adenine residues in DNA are oxidized under the action of ionizing radiation at the C-8 position to give 7,8-dihydro-8-oxoadenine. The formation of this lesion can be considered a cause of mutations and carcinogenesis. Oligodeoxyribonucleotides 39 and 47 bases long containing a single 7,8-dihydro-8-oxoadenine (8-hydroxyadenine) residue were synthesized by using nucleoside phosphoramidites. They were used as templates to study the copies obtained in vitro by the Klenow fragment and the thermostable Taq DNA polymerase. 7,8-Dihydro-8-oxoadenine does not block the replication and thymine is incorporated opposite the damage. The modifications of the DNA duplex conformation provoked by 7,8-dihydro-8-oxoadenine are minor. 1H-NMR spectroscopy shows that the duplex is in a B form, the sugar in a normal position in the helix and the modified base in the anti position. NMR confirms that 7,8-dihydro-8-oxoadenine exists predominantly in the keto form.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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