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. 1991 Apr 25;19(8):1811–1815. doi: 10.1093/nar/19.8.1811

A method of identifying and isolating a unique member of a multigene family: application to a trypanosome surface antigen gene.

B J Ruef 1, J H Hecht 1, J E Manning 1
PMCID: PMC328109  PMID: 2030963

Abstract

A chimeric oligonucleotide was constructed using DNA sequences from two distal regions of a cDNA which encodes a major surface antigen (TSA-1) of Trypanosoma cruzi. Conditions were found that allowed the chimeric oligonucleotide to hybridize only to a 5.4 kb EcoRI fragment in a Southern blot of total genomic DNA. The 5.4 kb EcoRI genomic DNA fragment has previously been shown to be located at a telomeric site, thus the studies described here directly demonstrate that the TSA-1 gene is telomeric in location. It is also shown that the chimeric oligonucleotide can be used to selectively identify recombinant lambda phage which harbor the TSA-1 gene using standard library screening procedures. Since these studies demonstrate that a chimeric oligonucleotide can be used to identify in both Southern blots and library screens a single member among the more than sixty members of the TSA-1 gene family, it seems likely that chimeric oligonucleotides may be of general use in studies involving repetitive DNA sequence families.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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