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. 1991 Apr 25;19(8):1825–1829. doi: 10.1093/nar/19.8.1825

Overexpression, purification and crystallization of BamHI endonuclease.

W E Jack 1, L Greenough 1, L F Dorner 1, S Y Xu 1, T Strzelecka 1, A K Aggarwal 1, I Schildkraut 1
PMCID: PMC328111  PMID: 2030964

Abstract

The type II restriction endonuclease BamHI has been expressed in E. coli, producing 100-fold more enzyme than the wild type Bacillus amyloliquefaciens H strain. This high yield has facilitated purification to homogeneity of large amounts of the enzyme, along with its crystallization in a form which diffracts to at least 1.9 A in X-ray analysis.

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Selected References

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