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. 1991 Apr 25;19(8):1879–1884. doi: 10.1093/nar/19.8.1879

A simple method for quantifying specific mRNAs in small numbers of early mouse embryos.

M F Gaudette 1, W R Crain 1
PMCID: PMC328118  PMID: 1903203

Abstract

Amplification of sequences by the polymerase chain reaction (PCR) has become a powerful tool in the study of gene expression. The technique is, in fact, so powerful that it may detect 'leaky transcription'. Thus, it is now important to be able to quantify the transcripts that are amplified to determine whether or not they represent legitimate transcription of target genes. In this paper, we describe a one-step amplification reaction coupled to solution hybridization/RNase protection that is capable of quantitating specific transcripts in total RNA from one to ten preimplantation mouse embryos and is generally applicable to any cloned mRNA sequence.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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