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. 1991 Apr 25;19(8):1948. doi: 10.1093/nar/19.8.1948

Rapid and simple purification of T7 RNA polymerase.

V Zawadzki 1, H J Gross 1
PMCID: PMC328134  PMID: 2030975

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Davanloo P., Rosenberg A. H., Dunn J. J., Studier F. W. Cloning and expression of the gene for bacteriophage T7 RNA polymerase. Proc Natl Acad Sci U S A. 1984 Apr;81(7):2035–2039. doi: 10.1073/pnas.81.7.2035. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Grodberg J., Dunn J. J. ompT encodes the Escherichia coli outer membrane protease that cleaves T7 RNA polymerase during purification. J Bacteriol. 1988 Mar;170(3):1245–1253. doi: 10.1128/jb.170.3.1245-1253.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Milligan J. F., Uhlenbeck O. C. Synthesis of small RNAs using T7 RNA polymerase. Methods Enzymol. 1989;180:51–62. doi: 10.1016/0076-6879(89)80091-6. [DOI] [PubMed] [Google Scholar]
  4. Sampson J. R., Uhlenbeck O. C. Biochemical and physical characterization of an unmodified yeast phenylalanine transfer RNA transcribed in vitro. Proc Natl Acad Sci U S A. 1988 Feb;85(4):1033–1037. doi: 10.1073/pnas.85.4.1033. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Szewczak A. A., White S. A., Gewirth D. T., Moore P. B. On the use of T7 RNA polymerase transcripts for physical investigation. Nucleic Acids Res. 1990 Jul 25;18(14):4139–4142. doi: 10.1093/nar/18.14.4139. [DOI] [PMC free article] [PubMed] [Google Scholar]

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