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. 1991 Jun 11;19(11):2935–2940. doi: 10.1093/nar/19.11.2935

A convenient approach to the synthesis of medium size oligodeoxyribonucleotides by improved new phosphite method.

H Hosaka 1, Y Suzuki 1, H Sato 1, S Gug-Kim 1, H Takaku 1
PMCID: PMC328254  PMID: 2057352

Abstract

Improvement of the new phosphite method for the synthesis of oligodeoxyribonucleotides using the deoxyribonucleoside 3'-bis(1,1,1,3,3,3- hexafluoro-2-propyl) phosphite unit has been carried out via the hydrolysis and capping steps, without any side reaction products. The new phosphite unit and capping agent, bis(1,1,1,3,3,3-hexafluoro-2-propyl)-2-propyl phosphite, is readily activated by N-methylimdazole under very mild condition on a solid support. This operation involves a one pot reaction, which is an advantage over both the phosphite and H- phosphonate approaches. The mechanism of internucleotidic bond formation of the new phosphite method is also discussed.

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Selected References

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