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. 1991 Jun 11;19(11):3139–3141. doi: 10.1093/nar/19.11.3139

Eliminating primers from completed polymerase chain reactions with exonuclease VII.

H H Li 1, X F Cui 1, N Arnheim 1
PMCID: PMC328282  PMID: 2057368

Abstract

Single-stranded oligonucleotide primers can be efficiently removed after PCR using E.coli exonuclease VII. Even only a few molecules of double stranded PCR product are unaffected by a treatment which eliminates 20 picomoles of primer in the presence of 500 ng of denatured genomic DNA. Exonuclease VII treatment is rapid and could simplify complicated multistep PCR protocols.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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