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. 1991 Jun 11;19(11):3153. doi: 10.1093/nar/19.11.3153

Temperature sweep gel electrophoresis: a simple method to detect point mutations.

K Yoshino 1, K Nishigaki 1, Y Husimi 1
PMCID: PMC328288  PMID: 2057372

Abstract

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Fischer S. G., Lerman L. S. Length-independent separation of DNA restriction fragments in two-dimensional gel electrophoresis. Cell. 1979 Jan;16(1):191–200. doi: 10.1016/0092-8674(79)90200-9. [DOI] [PubMed] [Google Scholar]
  2. Husimi Y. Selection and evolution of bacteriophages in cellstat. Adv Biophys. 1989;25:1–43. doi: 10.1016/0065-227x(89)90003-8. [DOI] [PubMed] [Google Scholar]
  3. Nishigaki K., Husimi Y., Tsubota M. Detection of differences in higher order structure between highly homologous single-stranded DNAs by low-temperature denaturant gradient gel electrophoresis. J Biochem. 1986 Mar;99(3):663–671. doi: 10.1093/oxfordjournals.jbchem.a135525. [DOI] [PubMed] [Google Scholar]
  4. Po T., Steger G., Rosenbaum V., Kaper J., Riesner D. Double-stranded cucumovirus associated RNA 5: experimental analysis of necrogenic and non-necrogenic variants by temperature-gradient gel electrophoresis. Nucleic Acids Res. 1987 Jul 10;15(13):5069–5083. doi: 10.1093/nar/15.13.5069. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Sheffield V. C., Cox D. R., Lerman L. S., Myers R. M. Attachment of a 40-base-pair G + C-rich sequence (GC-clamp) to genomic DNA fragments by the polymerase chain reaction results in improved detection of single-base changes. Proc Natl Acad Sci U S A. 1989 Jan;86(1):232–236. doi: 10.1073/pnas.86.1.232. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Steger G., Po T., Kaper J., Riesner D. Double-stranded cucumovirus associated RNA 5: which sequence variations may be detected by optical melting and temperature-gradient gel electrophoresis? Nucleic Acids Res. 1987 Jul 10;15(13):5085–5103. doi: 10.1093/nar/15.13.5085. [DOI] [PMC free article] [PubMed] [Google Scholar]

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