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. 1991 Jun 25;19(12):3193–3198. doi: 10.1093/nar/19.12.3193

Antisense probes containing 2-aminoadenosine allow efficient depletion of U5 snRNP from HeLa splicing extracts.

G M Lamm 1, B J Blencowe 1, B S Sproat 1, A M Iribarren 1, U Ryder 1, A I Lamond 1
PMCID: PMC328310  PMID: 1648201

Abstract

RNA duplexes containing the modified base 2-amino-adenine in place of adenine are stabilized through the formation of three hydrogen bonds in 2-amino A.U base pairs. Antisense 2'-O-alkyloligoribonucleotide probes incorporating 2-aminoadenosine are thus able to efficiently affinity select RNP particles which are otherwise inaccessible. This has allowed the efficient and specific depletion of U5 snRNP from HeLa cell nuclear splicing extracts. U5 snRNP is shown to be essential for spliceosome assembly and for both steps of pre-mRNA splicing. The absence of U5 snRNP prevents the stable association of U4/U6 but not U1 and U2 snRNPs with pre-mRNA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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