Skip to main content
NCBI home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1991 Jun 25;19(12):3207–3211. doi: 10.1093/nar/19.12.3207

Isolation and genetic structure of the AvaII isoschizomeric restriction-modification system HgiBI from Herpetosiphon giganteus Hpg5: M.HgiBI reveals high homology to M.BanI.

A Düsterhöft 1, D Erdmann 1, M Kröger 1
PMCID: PMC328312  PMID: 2062638

Abstract

The complete type II restriction-modification system HgiBI of Herpetosiphon giganteus strain Hpg5 recognizing the AvaII specific DNA sequence GGWCC has been cloned and expressed functionally active in Escherichia coli. A considerable acceleration in cloning could be achieved by preparing a size restricted library after application of a related hybridization probe. Both methyltransferase (437 codons) and restriction endonuclease gene (274 codons) were found to be encoded on a 3.6 kilobases ClaI/HincII fragment in the same transcriptional orientation separated by one triplett only. Protein sequence comparisons revealed a close resemblance of M.HgiBI to the group of m5C-methyltransferases, especially to M.BanI from Bacillus aneurinolyticus with the related recognition sequence GGYRCC. In contrast, no significant similarities have been observed for the associated endonuclease R.HgiBI with any other restriction enzyme described so far, even not with the isoschizomeric R.SinI from Salmonella infantis, or with R.BanI.

Full text

PDF
3207

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Düsterhöft A., Erdmann D., Kröger M. Stepwise cloning and molecular characterization of the HgiDI restriction-modification system from Herpetosiphon giganteus Hpa2. Nucleic Acids Res. 1991 Mar 11;19(5):1049–1056. doi: 10.1093/nar/19.5.1049. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Hanahan D. Studies on transformation of Escherichia coli with plasmids. J Mol Biol. 1983 Jun 5;166(4):557–580. doi: 10.1016/s0022-2836(83)80284-8. [DOI] [PubMed] [Google Scholar]
  3. Karreman C., de Waard A. Cloning and complete nucleotide sequences of the type II restriction-modification genes of Salmonella infantis. J Bacteriol. 1988 Jun;170(6):2527–2532. doi: 10.1128/jb.170.6.2527-2532.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Kessler C., Manta V. Specificity of restriction endonucleases and DNA modification methyltransferases a review (Edition 3). Gene. 1990 Aug 16;92(1-2):1–248. doi: 10.1016/0378-1119(90)90486-b. [DOI] [PubMed] [Google Scholar]
  5. Kröger M., Hobom G., Schütte H., Mayer H. Eight new restriction endonucleases fröm Herpetosiphon giganteus--divergent evolution in a family of enzymes. Nucleic Acids Res. 1984 Apr 11;12(7):3127–3141. doi: 10.1093/nar/12.7.3127. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kröger M., Kröger-Block A. Extension of a flexible computer program for handling DNA sequence data. Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):113–120. doi: 10.1093/nar/12.1part1.113. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Lauster R. Evolution of type II DNA methyltransferases. A gene duplication model. J Mol Biol. 1989 Mar 20;206(2):313–321. doi: 10.1016/0022-2836(89)90481-6. [DOI] [PubMed] [Google Scholar]
  8. Lauster R., Trautner T. A., Noyer-Weidner M. Cytosine-specific type II DNA methyltransferases. A conserved enzyme core with variable target-recognizing domains. J Mol Biol. 1989 Mar 20;206(2):305–312. doi: 10.1016/0022-2836(89)90480-4. [DOI] [PubMed] [Google Scholar]
  9. Maekawa Y., Yasukawa H., Kawakami B. Cloning and nucleotide sequences of the BanI restriction-modification genes in Bacillus aneurinolyticus. J Biochem. 1990 Apr;107(4):645–649. doi: 10.1093/oxfordjournals.jbchem.a123101. [DOI] [PubMed] [Google Scholar]
  10. Mayer H., Reichenbach H. Restriction endonucleases: general survey procedure and survey of gliding bacteria. J Bacteriol. 1978 Nov;136(2):708–713. doi: 10.1128/jb.136.2.708-713.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Pósfai J., Bhagwat A. S., Pósfai G., Roberts R. J. Predictive motifs derived from cytosine methyltransferases. Nucleic Acids Res. 1989 Apr 11;17(7):2421–2435. doi: 10.1093/nar/17.7.2421. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Roberts R. J. Restriction enzymes and their isoschizomers. Nucleic Acids Res. 1990 Apr 25;18 (Suppl):2331–2365. doi: 10.1093/nar/18.suppl.2331. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Szilák L., Venetianer P., Kiss A. Cloning and nucleotide sequence of the genes coding for the Sau96I restriction and modification enzymes. Nucleic Acids Res. 1990 Aug 25;18(16):4659–4664. doi: 10.1093/nar/18.16.4659. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Szomolányi E., Kiss A., Venetianer P. Cloning the modification methylase gene of Bacillus sphaericus R in Escherichia coli. Gene. 1980 Aug;10(3):219–225. doi: 10.1016/0378-1119(80)90051-7. [DOI] [PubMed] [Google Scholar]
  16. Tommasino M., Ricci S., Galeotti C. L. Genome organization of the killer plasmid pGK12 from Kluyveromyces lactis. Nucleic Acids Res. 1988 Jul 11;16(13):5863–5878. doi: 10.1093/nar/16.13.5863. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Wilson G. G. Cloned restriction-modification systems--a review. Gene. 1988 Dec 25;74(1):281–289. doi: 10.1016/0378-1119(88)90304-6. [DOI] [PubMed] [Google Scholar]
  18. Wilson G. G. Type II restriction--modification systems. Trends Genet. 1988 Nov;4(11):314–318. doi: 10.1016/0168-9525(88)90109-6. [DOI] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES