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. 2012 Mar 15;139(6):1141–1152. doi: 10.1242/dev.072439

Fig. 1.

Fig. 1.

sensory-deprived (sdp) mutants exhibit a complete loss of DRG sensory neurons. (A) Tg(neurog1:egfp) zebrafish embryos (3 dpf) immunostained for Elavl1. (A′) DRG are absent in an sdpw12/w12 mutant embryo. Scale bar: 500 μm. (B,B′) High magnification images of A and A′. Scale bar: 50 μm. (C,C′) neurog1 expression at 48 hpf. Box indicates position of high magnification images in D,D′. Scale bar: 250 μm. (D,D′) High magnification image of C and C′. Scale bar: 50 μm. Arrowheads indicate neurog+ DRG sensory neuron precursors. (E,E′) Tg(sox10:eos) embryo (3 dpf) immunostained for Elavl1. Although Schwann glia (empty arrowhead) are retained, satellite glia (filled arrowhead) are absent in sdpw12/w12. Scale bar: 25 μm. (F,F′) Counts of neurog1+ DRG at 3 dpf. Approximately 25% of embryos fail to form DRG, suggesting that sdpw12 is a fully penetrant recessive mutation. (G) Counts of neurog1+ DRG followed over four days. DRG never appear in ∼25% of the population, indicating that sdp does not delay in DRG development.