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. 1991 Jun 25;19(12):3237–3241. doi: 10.1093/nar/19.12.3237

In situ hybridization with fluoresceinated DNA.

J Wiegant 1, T Ried 1, P M Nederlof 1, M van der Ploeg 1, H J Tanke 1, A K Raap 1
PMCID: PMC328316  PMID: 2062640

Abstract

We have used fluorescein-11-dUTP in a nick-translation format to produce fluoresceinated human nucleic acid probes. After in situ hybridization of fluoresceinated DNAs to human metaphase chromosomes, the detection sensitivity was found to be 50-100 kb. The feasibility and the increase in detection sensitivity of microscopic imaging of in situ hybridized, fluoresceinated DNA with an integrating solid state camera for rapid cosmid mapping is illustrated. Combination of fluoresceinated DNA with biotinated and digoxigeninated DNAs allowed easy performance of triple fluorescence in situ hybridization. The potential of these techniques for DNA mapping, cytogenetics and biological dosimetry is briefly discussed.

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Selected References

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