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. 1991 Jul 11;19(13):3593–3599. doi: 10.1093/nar/19.13.3593

Termination of transcription in an 'in vitro' system is dependent on a polyadenylation sequence.

V J Miralles 1
PMCID: PMC328384  PMID: 1712938

Abstract

Using HeLa cell nuclear extract as a source of the different transcription and polyadenylation factors and reverse transcription to analyze the levels of RNA 5' and 3' to the cleavage-polyadenylation site, an in vitro assay has been established to study polyadenylation coupled to transcription directed by different adenovirus promoters. The levels of transcription 5' and 3' to the cleavage site in the L3 polyadenylation region are practically the same as described previously, however, the level of transcription 3' to the cleavage site in the SV40 early polyadenylation region decreases immediately after the cleavage site indicating a termination of the transcription.

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Selected References

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